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Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing

Authors
Park, TM; Kang, D; Jang, I; Yun, WS; Shim, JH; Jeong, YH; Kwak, JY; Yoon, S; Jin, S
Citation
International journal of molecular sciences, 18(11):E2348-E2348, 2017
Journal Title
International journal of molecular sciences
ISSN
1422-0067
Abstract
In general, a drug candidate is evaluated using 2D-cultured cancer cells followed by an animal model. Despite successful preclinical testing, however, most drugs that enter human clinical trials fail. The high failure rates are mainly caused by incompatibility between the responses of the current models and humans. Here, we fabricated a cancer microtissue array in a multi-well format that exhibits heterogeneous and batch-to-batch structure by continuous deposition of collagen-suspended Hela cells on a fibroblast-layered nanofibrous membrane via inkjet printing. Expression of both Matrix Metalloproteinase 2 (MMP2) and Matrix Metalloproteinase 9 (MMP9) was higher in cancer microtissues than in fibroblast-free microtissues. The fabricated microtissues were treated with an anticancer drug, and high drug resistance to doxorubicin occurred in cancer microtissues but not in fibroblast-free microtissues. These results introduce an inkjet printing fabrication method for cancer microtissue arrays, which can be used for various applications such as early drug screening and gradual 3D cancer studies.
MeSH terms
Cell SurvivalFibroblasts/cytology*HeLa CellsHumansMatrix Metalloproteinase 2/metabolismMatrix Metalloproteinase 9/metabolismNanofibers/chemistry*Tissue Array Analysis/methods
DOI
10.3390/ijms18112348
PMID
29112150
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
AJOU Authors
곽, 종영
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