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Low-Level Light Therapy with 410 nm Light Emitting Diode Suppresses Collagen Synthesis in Human Keloid Fibroblasts: An In Vitro Study

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dc.contributor.authorLee, HS-
dc.contributor.authorJung, SE-
dc.contributor.authorKim, SK-
dc.contributor.authorKim, YS-
dc.contributor.authorSohn, S-
dc.contributor.authorKim, YC-
dc.date.accessioned2018-08-24T01:50:22Z-
dc.date.available2018-08-24T01:50:22Z-
dc.date.issued2017-
dc.identifier.issn1013-9087-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/16170-
dc.description.abstractBACKGROUND: Keloids are characterized by excessive collagen deposition in the dermis, in which transforming growth factor beta (TGF-beta)/Smad signaling plays an important role. Low-level light therapy (LLLT) is reported as effective in preventing keloids in clinical reports, recently. To date, studies investigating the effect of LLLT on keloid fibroblasts are extremely rare.
OBJECTIVE: We investigated the effect of LLLT with blue (410 nm), red (630 nm), and infrared (830 nm) light on the collagen synthesis in keloid fibroblasts.
METHODS: Keloid fibroblasts were isolated from keloid-revision surgery samples and irradiated using 410-, 630-, 830-nm light emitting diode twice, with a 24-hour interval at 10 J/cm(2). After irradiation, cells were incubated for 24 and 48 hours and real-time quantitative reverse transcription polymerase chain reaction was performed. Western blot analysis was also performed in 48 hours after last irradiation. The genes and proteins of collagen type I, TGF-beta1, Smad3, and Smad7 were analyzed.
RESULTS: We observed no statistically significant change in the viability of keloid fibroblasts after irradiation. Collagen type I was the only gene whose expression significantly decreased after irradiation at 410 nm when compared to the non-irradiated control. Western blot analysis showed that LLLT at 410 nm lowered the protein levels of collagen type I compared to the control.
CONCLUSION: LLLT at 410 nm decreased the expression of collagen type I in keloid fibroblasts and might be effective in preventing keloid formation in their initial stage.
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dc.language.isoen-
dc.titleLow-Level Light Therapy with 410 nm Light Emitting Diode Suppresses Collagen Synthesis in Human Keloid Fibroblasts: An In Vitro Study-
dc.typeArticle-
dc.identifier.pmid28392641-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383739/-
dc.subject.keywordCollagen type I-
dc.subject.keywordKeloid fibroblast-
dc.subject.keywordLow-level light therapy-
dc.contributor.affiliatedAuthor이, 현수-
dc.contributor.affiliatedAuthor김, 유선-
dc.contributor.affiliatedAuthor손, 성향-
dc.contributor.affiliatedAuthor김, 유찬-
dc.type.localJournal Papers-
dc.identifier.doi10.5021/ad.2017.29.2.149-
dc.citation.titleAnnals of dermatology-
dc.citation.volume29-
dc.citation.number2-
dc.citation.date2017-
dc.citation.startPage149-
dc.citation.endPage155-
dc.identifier.bibliographicCitationAnnals of dermatology, 29(2). : 149-155, 2017-
dc.identifier.eissn2005-3894-
dc.relation.journalidJ010139087-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Dermatology
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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