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Transcription Factors Regulating Inflammatory Cytokine Production Are Differentially Expressed in Peripheral Blood Mononuclear Cells of Behcet Disease Depending on Disease Activity

Authors
Woo, MY | Yun, SJ | Lee, MJ  | Kim, K  | Lee, ES  | Park, S
Citation
Annals of dermatology, 29(2). : 173-179, 2017
Journal Title
Annals of dermatology
ISSN
1013-90872005-3894
Abstract
BACKGROUND: Behcet disease (BD) is a relapsing inflammatory disease with increased production of inflammatory cytokines in peripheral blood mononuclear cells (PBMCs): however, the underlying molecular mechanisms are not well known.
OBJECTIVE: To analyze whether the differential expression of transcription factors is involved in the increased tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 production by PBMCs of BD patients compared to healthy controls (HCs).
METHODS: Expression of transcription factors was examined by real-time reverse transcriptase-polymerase chain reaction and western blotting. Cytokine production by CD11b+ cells transfected with siRNAs against transcription factors was measured by enzyme-linked immunosorbent assay.
RESULTS: In the absence of lipopolysaccharide stimulation, the transcript level of CCAAT-enhancer-binding proteins (C/EBP) beta was increased in PBMCs from patients with active BD compared to that in PBMCs from patients with stable BD. The C/EBPdelta transcript level was higher in PBMCs from patients with active BD than in those from HCs. The activating transcription factor 3 (ATF3) transcript level was increased in PBMCs from patients with stable BD compared to that in PBMCs from HCs. siRNAs targeting C/EBPbeta and C/EBPdelta significantly reduced the production of IL-6 and TNF-alpha in lipopolysaccharide-stimulated CD11b+ cells from patients with BD as well as from HCs.
CONCLUSION: We found differential expression of C/EBPbeta, C/EBPdelta, and ATF3 in PBMCs from patients with BD depending on disease activity, indicating the involvement of these molecules in BD pathogenesis.
Keywords

DOI
10.5021/ad.2017.29.2.173
PMID
28392644
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
Journal Papers > School of Medicine / Graduate School of Medicine > Dermatology
Ajou Authors
김, 경민  |  박, 선  |  이, 미진  |  이, 은소
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