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Simple Quantification of Pentosidine in Human Urine and Plasma by High-Performance Liquid Chromatography

Authors
Lee, JS | Chung, YS  | Chang, SY | Jung, YS | Kim, SH
Citation
International journal of analytical chemistry, 2017. : 1389807-1389807, 2017
Journal Title
International journal of analytical chemistry
ISSN
1687-87601687-8779
Abstract
Pentosidine is an advanced glycation end-product (AGE) and fluorescent cross-link compound. A simple high-performance liquid chromatographic (HPLC) method was developed for the detection and quantification of pentosidine in human urine and plasma. The mobile phase used a gradient system to improve separation of pentosidine from endogenous peaks, and chromatograms were monitored by fluorescent detector set at excitation and emission wavelengths of 328 and 378 nm, respectively. The retention time for pentosidine was 24.3 min and the lower limits of quantification (LLOQ) in human urine and plasma were 1 nM. The intraday assay precisions (coefficients of variation) were generally low and found to be in the range of 5.19-7.49% and 4.96-8.78% for human urine and plasma, respectively. The corresponding values of the interday assay precisions were 9.45% and 4.27%. Accuracies (relative errors) ranged from 87.9% to 115%. Pentosidine was stable in a range of pH solutions, human urine, and plasma. In summary, this HPLC method can be applied in future preclinical and clinical evaluation of pentosidine in the diabetic patients.
DOI
10.1155/2017/1389807
PMID
29181026
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Endocrinology & Metabolism
Ajou Authors
정, 윤석
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