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BMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells

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dc.contributor.authorPark, SH-
dc.contributor.authorKwon, JS-
dc.contributor.authorLee, BS-
dc.contributor.authorPark, JH-
dc.contributor.authorLee, BK-
dc.contributor.authorYun, JH-
dc.contributor.authorLee, BY-
dc.contributor.authorKim, JH-
dc.contributor.authorMin, BH-
dc.contributor.authorYoo, TH-
dc.contributor.authorKim, MS-
dc.date.accessioned2018-10-04T06:13:53Z-
dc.date.available2018-10-04T06:13:53Z-
dc.date.issued2017-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/16384-
dc.description.abstractThis is the first report on the development of a covalently bone morphogenetic protein-2 (BMP2)-immobilized hydrogel that is suitable for osteogenic differentiation of human periodontal ligament stem cells (hPLSCs). O-propargyl-tyrosine (OpgY) was site-specifically incorporated into BMP2 to prepare BMP2-OpgY with an alkyne group. The engineered BMP2-OpgY exhibited osteogenic characteristics after in vitro osteogenic differentiation of hPLSCs, indicating the osteogenic ability of BMP2-OpgY. A methoxy polyethylene glycol-(polycaprolactone-(N3)) block copolymer (MC-N3) was prepared as an injectable in situ-forming hydrogel. BMP2 covalently immobilized on an MC hydrogel (MC-BMP2) was prepared quantitatively by a simple biorthogonal reaction between alkyne groups on BMP2-OpgY and azide groups on MC-N3 via a Cu(I)-catalyzed click reaction. The hPLSCs-loaded MC-BMP2 formed a hydrogel almost immediately upon injection into animals. In vivo osteogenic differentiation of hPLSCs in the MC-BMP2 formulation was confirmed by histological staining and gene expression analyses. Histological staining of hPLSC-loaded MC-BMP2 implants showed evidence of mineralized calcium deposits, whereas hPLSC-loaded MC-Cl or BMP2-OpgY mixed with MC-Cl, implants showed no mineral deposits. Additionally, MC-BMP2 induced higher levels of osteogenic gene expression in hPLSCs than in other groups. In conclusion, BMP2-OpgY covalently immobilized on MC-BMP2 induced osteogenic differentiation of hPLSCs as a noninvasive method for bone tissue engineering.-
dc.language.isoen-
dc.titleBMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells-
dc.typeArticle-
dc.identifier.pmid28747761-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28747761/-
dc.contributor.localId101474-
dc.contributor.affiliatedAuthor민, 병현-
dc.type.localJournal Papers-
dc.identifier.doi10.1038/s41598-017-06911-8-
dc.identifier.localIdDOI-10.1038/s41598-017-06911-8-
dc.citation.titleScientific reports-
dc.citation.volume7-
dc.citation.number1-
dc.citation.date2017-
dc.citation.startPage6603-
dc.citation.endPage6603-
dc.identifier.bibliographicCitationScientific reports, 7(1):6603-6603, 2017-
dc.identifier.eissn20452322-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
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