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The rat vanilloid receptor splice variant VR.5'sv blocks TRPV1 activation.

Authors
Eilers, H; Lee, SY; Hau, CW; Logvinova, A; Schumacher, MA
Citation
Neuroreport, 18(10):969-973, 2007
Journal Title
Neuroreport
ISSN
0959-49651473-558X
Abstract
The capsaicin receptor (VR1, TRPV1) is a ligand-gated ion channel predominantly expressed in peripheral nociceptors and activated by multiple noxious stimuli including products of inflammation. A 5'-splice variant (VR.5'sv) of TRPV1 has been previously isolated and found to be insensitive to noxious stimuli. We report in this study that coexpression of VR.5'sv with TRPV1 in Xenopus oocytes blocks TRPV1-mediated current responses. Oocytes expressing the inhibitory profile demonstrated colocalization of TRPV1 and VR.5'sv-associated immunostaining in the plasma membrane. TRPV1 protein expression was comparable in all groups. Evidence of endogenous VR.5'-splice variant-like-protein expression was detected in dorsal root ganglion. These results support the idea that coexpression of VR.5'sv or a similar variant could result in inhibitory modulation of TRPV1 activation.
MeSH terms
Analysis of VarianceAnimalsBiotinylation/methodsGanglia, Spinal/cytologyGene ExpressionHot TemperatureMembrane Potentials/drug effectsMembrane Potentials/physiologyMicroinjectionsNeurons/drug effectsNeurons/physiologyOocytesPatch-Clamp TechniquesProtein Isoforms/metabolism*RatsTRPV Cation Channels/metabolism*Xenopus
DOI
10.1097/WNR.0b013e328165d1a2
PMID
17558279
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anesthesiology & Pain Medicine
AJOU Authors
이, 숙영
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