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Biological function of eosinophil extracellular traps in patients with severe eosinophilic asthma

Authors
Choi, Y; Le Pham, D; Lee, DH; Lee, SH; Kim, SH; Park, HS
Citation
Experimental & molecular medicine, 50(8):104-104, 2018
Journal Title
Experimental & molecular medicine
ISSN
1226-36132092-6413
Abstract
Eosinophil extracellular traps (EETs), a complex of DNA fibers and cytotoxic granule proteins, are implicated in the development of asthma: however, the pathophysiological function of EETs in immune responses has not been fully determined. The present study investigated the characteristics of EETs from patients with non-severe asthma (NSA, n = 20) and severe eosinophilic asthma (SEA, n = 20) and evaluated EET function. The percentage of EET-forming peripheral blood eosinophils stimulated with IL-5 and LPS was significantly higher in patients with SEA than in those with NSA (P = 0.009). This percentage negatively correlated with baseline FEV1 (r = -0.350, P = 0.027) and positively correlated with serum eosinophil-derived neurotoxin levels in asthmatic subjects (r = 0.437, P = 0.018). In addition, EET formation was markedly associated with reactive oxygen species production (r = 0.750, P < 0.001). These EETs exhibited an autocrine function to induce eosinophil degranulation, which led to granule protein production. Airway epithelial cells stimulated with EETs exhibited increased epithelial detachment and permeability and pro-inflammatory cytokine release. However, EETs were not significantly associated with mast cell activation. The present study suggests that peripheral blood eosinophils from patients with SEA may be more activated to produce EETs than those from patients with NSA, which further induces inflammation in asthmatic airways. Therefore, regulation of EET formation and function may be a novel therapeutic approach for asthma management.
MeSH terms
Asthma/bloodAsthma/immunologyAsthma/metabolism*Asthma/pathology*Autocrine CommunicationCell LineEosinophils/immunologyEosinophils/metabolism*Eosinophils/pathology*Epithelial Cells/immunologyEpithelial Cells/pathologyExtracellular Traps/metabolism*FemaleHumansLung/pathologyMaleMast Cells/metabolismMiddle AgedReactive Oxygen Species/metabolism
DOI
10.1038/s12276-018-0136-8
PMID
30115903
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Journal Papers > Research Organization > Regional Clinical Trial Center
AJOU Authors
이, 소희김, 승현박, 해심
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