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NAG-1 up-regulation mediated by EGR-1 and p53 is critical for quercetin-induced apoptosis in HCT116 colon carcinoma cells.

DC Field Value Language
dc.contributor.authorLim, JH-
dc.contributor.authorPark, JW-
dc.contributor.authorMin, DS-
dc.contributor.authorChang, JS-
dc.contributor.authorLee, YH-
dc.contributor.authorPark, YB-
dc.contributor.authorChoi, KS-
dc.contributor.authorKwon, TK-
dc.date.accessioned2011-03-16T06:13:02Z-
dc.date.available2011-03-16T06:13:02Z-
dc.date.issued2007-
dc.identifier.issn1360-8185-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/1750-
dc.description.abstractQuercetin, a flavonoid molecule ubiquitously present in nature, has multiple effects on cancer cells, including the inhibition of cell proliferation and migration. However, the responsible molecular mechanisms are not fully understood. We found that quercetin induces the expression of NAG-1 (Non-steroidal anti-inflammatory drug activated gene-1), a TGF-beta superfamily protein, during quercetin-induced apoptosis of HCT116 human colon carcinoma cells. Reporter assays using the luciferase constructs containing NAG-1 promoter region demonstrate that early growth response-1 (EGR-1) and p53 are required for quercetin-mediated activation of the NAG-1 promoter. Overexpression of NAG-1 enhanced the apoptotic effect of quercetin, but suppression of quercetin-induced NAG-1 expression by NAG-1 siRNA attenuated quercetin-induced apoptosis in HCT116 cells. Taken together, the present study demonstrates for the first time that quercetin induces apoptosis via NAG-1, providing a mechanistic basis for the apoptotic effect of quercetin in colon carcinoma cells.-
dc.language.isoen-
dc.subject.MESHApoptosis/drug effects*-
dc.subject.MESHBase Sequence-
dc.subject.MESHBinding Sites/drug effects-
dc.subject.MESHColonic Neoplasms/genetics-
dc.subject.MESHColonic Neoplasms/pathology*-
dc.subject.MESHCytokines/genetics*-
dc.subject.MESHCytokines/metabolism-
dc.subject.MESHDose-Response Relationship, Drug-
dc.subject.MESHEarly Growth Response Protein 1/metabolism*-
dc.subject.MESHGene Expression Regulation, Neoplastic/drug effects-
dc.subject.MESHGrowth Differentiation Factor 15-
dc.subject.MESHHCT116 Cells-
dc.subject.MESHHT29 Cells-
dc.subject.MESHHumans-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPromoter Regions, Genetic/drug effects-
dc.subject.MESHPromoter Regions, Genetic/genetics-
dc.subject.MESHQuercetin/pharmacology*-
dc.subject.MESHRNA, Messenger/genetics-
dc.subject.MESHRNA, Messenger/metabolism-
dc.subject.MESHSp1 Transcription Factor/metabolism-
dc.subject.MESHTranscriptional Activation/drug effects-
dc.subject.MESHTumor Suppressor Protein p53/metabolism*-
dc.subject.MESHUp-Regulation/drug effects*-
dc.titleNAG-1 up-regulation mediated by EGR-1 and p53 is critical for quercetin-induced apoptosis in HCT116 colon carcinoma cells.-
dc.typeArticle-
dc.identifier.pmid17191121-
dc.contributor.affiliatedAuthor최, 경숙-
dc.type.localJournal Papers-
dc.identifier.doi10.1007/s10495-006-0576-9-
dc.citation.titleApoptosis : an international journal on programmed cell death-
dc.citation.volume12-
dc.citation.number2-
dc.citation.date2007-
dc.citation.startPage411-
dc.citation.endPage421-
dc.identifier.bibliographicCitationApoptosis : an international journal on programmed cell death, 12(2):411-421, 2007-
dc.identifier.eissn1573-675X-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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