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Carboxylate-Containing Two-Photon Probe for the Simultaneous Detection of Extra- and Intracellular pH Values in Colon Cancer Tissue

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dc.contributor.authorSi, HY-
dc.contributor.authorCho, MK-
dc.contributor.authorKang, JS-
dc.contributor.authorNoh, CK-
dc.contributor.authorShin, SJ-
dc.contributor.authorLim, CS-
dc.contributor.authorKim, HM-
dc.date.accessioned2019-11-13T04:27:55Z-
dc.date.available2019-11-13T04:27:55Z-
dc.date.issued2018-
dc.identifier.issn0003-2700-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/17634-
dc.description.abstractAcidified extracellular pH (pHe) is directly related to various disorders such as tumor invasion and the resistance to drugs. In this study, we developed two-photon-excitable emission ratiometric probes (XBH1-3) for the in situ measurement of pHe. These probes, based on benzimidazole and polar solubilizing groups, exhibited a strong two-photon-induced fluorescence and sensitive blue-to-green emission color changes with p Ka values of 5.1-5.7. XBH1, containing a carboxylic acid, stained the extracellular region in neutral media: it entered the cell under acidic media, thereby allowing a precise measurement of the extra- and intra-cellular pH values in the acidified tissue. XBH2, containing the sulfonate peripheral unit, facilitated the monitoring of the pHe value only. Ratiometric two-photon microscopy imaging revealed that XBH1 can directly monitor the pH values both inside and outside the cells in colon cancer tissue: there is also the morphological aspect. This could be useful for cancer analyses and drug development.-
dc.language.isoen-
dc.subject.MESHCarboxylic Acids/chemistry-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHColonic Neoplasms/pathology-
dc.subject.MESHExtracellular Space/chemistry-
dc.subject.MESHFluorescent Dyes/chemistry-
dc.subject.MESHHeLa Cells-
dc.subject.MESHHumans-
dc.subject.MESHHydrogen-Ion Concentration-
dc.subject.MESHIntracellular Space/chemistry-
dc.subject.MESHPhotons-
dc.subject.MESHSolubility-
dc.subject.MESHSpectrometry, Fluorescence-
dc.subject.MESHSulfonic Acids/chemistry-
dc.subject.MESHTime Factors-
dc.titleCarboxylate-Containing Two-Photon Probe for the Simultaneous Detection of Extra- and Intracellular pH Values in Colon Cancer Tissue-
dc.typeArticle-
dc.identifier.pmid29847925-
dc.contributor.affiliatedAuthor노, 충균-
dc.contributor.affiliatedAuthor신, 성재-
dc.type.localJournal Papers-
dc.identifier.doi10.1021/acs.analchem.8b01114-
dc.citation.titleAnalytical chemistry-
dc.citation.volume90-
dc.citation.number13-
dc.citation.date2018-
dc.citation.startPage8058-
dc.citation.endPage8064-
dc.identifier.bibliographicCitationAnalytical chemistry, 90(13). : 8058-8064, 2018-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.identifier.eissn1520-6882-
dc.relation.journalidJ000032700-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
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