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Effect of an oxygen-generating scaffold on the viability and insulin secretion function of porcine neonatal pancreatic cell clusters

Authors
Lee, EM; Jung, JI; Alam, Z; Yi, HG; Kim, H; Choi, JW; Hurh, S; Kim, YJ; Jeong, JC; Yang, J; Oh, KH; Kim, HC; Lee, BC; Choi, I; Cho, DW; Ahn, C
Citation
Xenotransplantation, 25(2):e12378-e12378, 2018
Journal Title
Xenotransplantation
ISSN
0908-665X1399-3089
Abstract
BACKGROUND: Islet encapsulation techniques have shown limited success in maintaining islet survival and function because encapsulation decreases oxygen supply. In this study, an oxygen-generating scaffold was fabricated to prevent hypoxic cell damage and improve the viability and insulin secretion of islets.
METHODS: We fabricated an oxygen-generating scaffold by mixing calcium peroxide (CaO2 ) with polydimethylsiloxane (PDMS). We evaluated the effects of the oxygen-generating PDMS + CaO2 scaffold on viability, caspase-3 and caspase-7 activity, oxygen consumption rate (OCR), glucose-stimulated insulin secretion (GSIS), hypoxic cell marker expression, and reactive oxygen species (ROS) levels in porcine neonatal pancreatic cell clusters (NPCCs). We also fabricated a microfluidic device that allowed measuring the effects of the oxygen-generating scaffold on viability.
RESULTS: Oxygen generation by the PDMS + CaO2 scaffold was sustained for more than 24 hours in vitro. NPCCs encapsulated in PDMS + CaO2 showed higher viability than NPCCs in PDMS scaffolds and control NPCCs grown without a scaffold. PDMS + CaO2 -encapsulated NPCCs showed lower caspase-3 and caspase-7 activity, hypoxic cell expression, and ROS levels, and higher OCR and GSIS than those in PDMS or control cells. Using the microfluidic device, we observed that the viability of PDMS + CaO2 -encapsulated NPCCs was higher than that of PDMS-encapsulated NPCCs.
CONCLUSIONS: NPCCs in PDMS + CaO2 scaffolds show higher viability and insulin secretion than do NPCCs in PDMS scaffolds and control cells. Therefore, this oxygen-generating scaffold has potential for application in future islet transplantation studies.
Keywords
insulin secretionmacro-encapsulationmicrofluidic systemneonatal pancreatic cell clustersoxygen-generating scaffold
MeSH terms
AnimalsAnimals, NewbornBlood Glucose/metabolismCell Survival/physiology*Diabetes Mellitus, ExperimentalInsulin/metabolism*Insulin SecretionIslets of Langerhans Transplantation*/methodsOxygen/metabolism*Pancreas/metabolismSwineTransplantation, Heterologous/methods
DOI
10.1111/xen.12378
PMID
29322561
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Nephrology
AJOU Authors
정, 종철
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