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Dual-functionalized calcium nanocomplexes for transfection of cancerous and stem cells: Low molecular weight polycation-mediated colloidal stability and ATP-mediated endosomal release

Authors
Choi, YS | Kim, K | Ryu, K | Cho, H | Cho, YY | Lee, JY | Lee, HS | Kim, BG  | Song, SC | Kang, HC
Citation
Journal of industrial and engineering chemistry (Seoul, Korea), 64. : 300-310, 2018
Journal Title
Journal of industrial and engineering chemistry (Seoul, Korea)
ISSN
1226-086X1876-794X
Abstract
To overcome colloidal instability of calcium phosphate nanoparticles in gene delivery, colloidally stable and endosomolytic Ca2+-based pDNA nanocomplexes (NCs) were designed by a surface coating of biocompatible polycations (PCs; low molecular weight branched polyethyleneimine [bPEI], protamine sulfate and ε-polylysine) and the addition of natural and endosomolytic ATP, respectively. Without remarkable cytotoxicity and colloidal instability, Ca2+/ATP-pDNA/bPEI1.8 kDa NCs having [bPEI1.8 kDa] = 3.6 μg showed 5.8-fold and 4.4-fold higher transfection efficiencies than bPEI25 kDa/pDNA NCs in HepG2 cells and dental pulp stem cells, respectively. In conclusion, pH-sensitive endosomolytic ATP and Ca2+-based gene complexes could be potentials as effective and safe gene delivery vectors in various cells.
Keywords
DOI
10.1016/j.jiec.2018.03.028
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Brain Science
Ajou Authors
김, 병곤
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