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Recapitulation of cancer stem cell niches in glioblastoma on 3D microfluidic cell culture devices under gravity-driven perfusion

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dc.contributor.authorHan, J-
dc.contributor.authorOh, S-
dc.contributor.authorHoang, HH-
dc.contributor.authorNguyen, DTT-
dc.contributor.authorLim, W-
dc.contributor.authorShin, TH-
dc.contributor.authorLee, G-
dc.contributor.authorPark, S-
dc.date.accessioned2019-12-10T06:53:39Z-
dc.date.available2019-12-10T06:53:39Z-
dc.date.issued2018-
dc.identifier.issn1226-086X-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/17784-
dc.description.abstractThe low survival rate of patients with glioblastoma is in part due to the heterogeneity in the cell population of glioblastoma that includes cancer stem cells (CSCs). CSC niches include a hypoxic core that is also closely linked to self-renewal ability, migration, and drug resistance. Here, we report a CSC culture method in three-dimensional microfluidic cell culture devices under gravity-driven perfusion, which we dub hypoxia chips (H-chips). In H-chips, glioblastoma cells, U87, spontaneously formed spheroids within 12 h, even without any addition of growth factors. Compared to monolayer-cultured cells in dishes, spheroids in H-chips showed higher expression of CSC markers, such as hypoxia-inducible factor-1α (HIF-1α), CD133, and nestin. Spheroids in H-chips were more resistant to doxorubicin than monolayer-cultured ones in dishes. Transcriptional profiling revealed that the expression of interleukin-6 (IL-6), one of the inflammatory cytokines, was higher in spheroids in H-chips than in monolayer-cultured cells in dishes. IL-6 depleted cells failed to form spheroids in H-chips, and their drug resistance decreased. These results suggest that in H-chips, glioblastoma cells increased the production of IL-6, and promoted spheroid formation and other cancer stem cell properties, such as drug resistance. Our microfluidic cell culture method is highly useful for recapitulating CSC niches.-
dc.language.isoen-
dc.titleRecapitulation of cancer stem cell niches in glioblastoma on 3D microfluidic cell culture devices under gravity-driven perfusion-
dc.typeArticle-
dc.subject.keywordCancer stem cell-
dc.subject.keywordMicrofluidic cell culture-
dc.subject.keywordIL-6-
dc.subject.keywordSpheroid-
dc.subject.keywordDoxorubicin-
dc.contributor.affiliatedAuthor이, 광-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.jiec.2018.01.015-
dc.citation.titleJournal of industrial and engineering chemistry (Seoul, Korea)-
dc.citation.volume62-
dc.citation.date2018-
dc.citation.startPage352-
dc.citation.endPage361-
dc.identifier.bibliographicCitationJournal of industrial and engineering chemistry (Seoul, Korea), 62. : 352-361, 2018-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.identifier.eissn1876-794X-
dc.relation.journalidJ01226086X-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
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