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Inhibitory activities and attenuated expressions of 5-LOX with red ginseng in Helicobacter pylori-infected gastric epithelial cells.

Authors
Park, S; Yeo, M; Jin, JH; Lee, KM; Kim, SS; Choi, SY; Hahm, KB
Citation
Digestive diseases and sciences, 52(4):973-982, 2007
Journal Title
Digestive diseases and sciences
ISSN
0163-21161573-2568
Abstract
Our recent studies documented that red ginseng extract (RGE, isolates from steamed and dried Panax ginseng, C.A. Meyer) can inhibit Helicobacter pylori-induced mitogen-activated protein kinase (MAPK) signaling with repressing either nuclear factor (NF)-kappaB-DNA binding activity or releases of IL-8 and COX-2 in gastric epithelial cells (Dig Dis Sci 50:1218-1227, 2005). We extended the experiment to prove whether RGE influences 5-lipoxygenase (5-LOX) pathway, thereby suppressing the biosynthesis of 5(S)-HETE. The 5-LOX enzyme activities were measured by thin layer chromatography using (14)C-labeled arachidonic acid (AA) and quantified by reverse phase-high performance liquid chromatography in human gastric adenocarcinoma (AGS) cells cocultured with H pylori (ATCC 43504 strain) with or without pretreatment of RGE. Western blotting analyses for MAPK signaling and 5-LOX, reverse transcriptase polymerase chain reaction for interleukin-8, and electrophoretic mobility shift assay for NF-kappaB-DNA binding were done, respectively. H pylori infection increased exclusively 5-LOX enzyme activity and RGE inhibited H pylori-stimulated 5-LOX activity, resulting in suppression of 5(S)-HETE generations from AA. RGE inactivated c-jun phosphorylation and repressed redox-sensitive transcriptional activation, led to reduced expression of IL-8 and 5-LOX mRNA in gastric mucosal cells, of which action was very similar to known LOX inhibitor, 200 mumol of geraniin. RGE could be phytoceutical against H pylori infection-associated gastric inflammation through its LOX-inhibiting actions, inhibitory 5-LOX enzyme activity, and attenuating its expression.
MeSH terms
Adenocarcinoma/metabolismAdenocarcinoma/microbiologyArachidonate 5-Lipoxygenase/metabolism*Arachidonic Acid/metabolismChromatography, High Pressure LiquidChromatography, Thin LayerCyclooxygenase 2/metabolismEpithelial Cells/metabolism*Gastric Mucosa/metabolism*Gastric Mucosa/pathologyHelicobacter Infections/metabolism*Helicobacter Infections/pathologyHelicobacter pylori*HumansHydroxyeicosatetraenoic Acids/metabolismLipoxygenase InhibitorsMitogen-Activated Protein Kinases/metabolismPanax*Plant Extracts/pharmacology*Reverse Transcriptase Polymerase Chain ReactionSignal TransductionStomach Neoplasms/metabolismStomach Neoplasms/microbiologyTumor Cells, Cultured
DOI
10.1007/s10620-006-9440-6
PMID
17333352
Appears in Collections:
Journal Papers > Research Organization > Genomic Research Center for Gastroenterology
AJOU Authors
여, 말희
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