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Differential regulation of tyrosine hydroxylase expression by sonic hedgehog.

Authors
Kwon, IS; Park, RH; Choi, JM; Kim, SU; Lee, YD; Suh-Kim, H
Citation
Neuroreport, 17(7):693-698, 2006
Journal Title
Neuroreport
ISSN
0959-49651473-558X
Abstract
Sonic hedgehog functions to induce floor plate in early stages, and spinal motor neurons and midbrain dopaminergic neurons in later stages of development. Here, we investigated the effects of sonic hedgehog on tyrosine hydroxylase expression in three cell lines that correspond to different stages of neural development. Sonic hedgehog increased the tyrosine hydroxylase gene expression in pluripotent P19 cells but repressed it in tyrosine hydroxylase-producing PC12 cells. Promoter analysis in mouse neural stem cells indicated that the N-terminal of sonic hedgehog repressed both the basal and cAMP-dependent protein kinase A-mediated tyrosine hydroxylase activity. These results suggest that the N-terminal of sonic hedgehog increases tyrosine hydroxylase gene expression in cells to acquire dopaminergic phenotypes, but decreases expression in late born neurons by antagonizing the protein kinase A cAMP-responsive element binding protein pathway.
MeSH terms
AnimalsBlotting, Western/methodsCell Differentiation/physiologyCell LineCloning, Molecular/methodsGene Expression/drug effectsGene Expression/physiology*Hedgehog ProteinsRatsSignal Transduction/physiologyStem Cells/physiologyTrans-Activators/chemistryTrans-Activators/physiology*Transfection/methodsTyrosine 3-Monooxygenase/geneticsTyrosine 3-Monooxygenase/metabolism*
DOI
10.1097/01.wnr.0000209043.66482.0b
PMID
16641671
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
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