Cited 0 times in Scipus Cited Count

Inhibition of PP2A activity by H2O2 during mitosis disrupts nuclear envelope reassembly and alters nuclear shape

DC Field Value Language
dc.contributor.authorAhn, JH-
dc.contributor.authorCho, MG-
dc.contributor.authorSohn, S-
dc.contributor.authorLee, JH-
dc.date.accessioned2020-10-21T07:20:16Z-
dc.date.available2020-10-21T07:20:16Z-
dc.date.issued2019-
dc.identifier.issn1226-3613-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/18723-
dc.description.abstractMany types of cancer cells exhibit abnormal nuclear shapes induced by various molecular changes. However, whether reactive oxygen species (ROS) induce nuclear deformation has not been fully addressed. Here, we show that hydrogen peroxide (H2O2) treatment induced concentration-dependent alterations in nuclear shape that were abolished by pretreatment with the antioxidant N-acetyl-L-cysteine or by catalase overexpression. Interestingly, treatment with H2O2 induced nuclear shape alterations significantly more frequently in mitotic cells than in asynchronous cells, suggesting that H2O2 mainly affects nuclear envelope disassembly and/or reassembly processes. Because protein phosphatase 2 A (PP2A) activity is reported to be involved in nuclear envelope reassembly during mitosis, we investigated the possible involvement of PP2A. Indeed, H2O2 reduced the activity of PP2A, an effect that was mimicked by the PP1 and PP2A inhibitor okadaic acid. Moreover, overexpression of PP2A but not PP1 or PP4 partially rescued H2O2-induced alterations in nuclear shape, indicating that the decrease in PP2A activity induced by H2O2 is specifically involved in the observed nuclear shape alterations. We further show that treatment of mitotic cells with H2O2 induced the mislocalization of BAF (barrier-to-autointegration factor), a substrate of PP2A, during telophase. This effect was associated with Lamin A/C mislocalization and was rescued by PP2A overexpression. Collectively, our findings suggest that H2O2 preferentially affects mitotic cells through PP2A inhibition, which induces the subsequent mislocalization of BAF and Lamin A/C during nuclear envelope reassembly, leading to the formation of an abnormal nuclear shape.-
dc.language.isoen-
dc.subject.MESHCell Nucleus-
dc.subject.MESHEnzyme Activation-
dc.subject.MESHHeLa Cells-
dc.subject.MESHHumans-
dc.subject.MESHHydrogen Peroxide-
dc.subject.MESHMitosis-
dc.subject.MESHNuclear Envelope-
dc.subject.MESHProtein Phosphatase 2-
dc.titleInhibition of PP2A activity by H2O2 during mitosis disrupts nuclear envelope reassembly and alters nuclear shape-
dc.typeArticle-
dc.identifier.pmid31164634-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548778/-
dc.contributor.affiliatedAuthor손, 성향-
dc.contributor.affiliatedAuthor이, 재호-
dc.type.localJournal Papers-
dc.identifier.doi10.1038/s12276-019-0260-0-
dc.citation.titleExperimental & molecular medicine-
dc.citation.volume51-
dc.citation.date2019-
dc.citation.startPage64-
dc.citation.endPage64-
dc.identifier.bibliographicCitationExperimental & molecular medicine, 51. : 64-64, 2019-
dc.identifier.eissn2092-6413-
dc.relation.journalidJ012263613-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
Files in This Item:
31164634.pdfDownload

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse