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Mitochondria are impaired in the adipocytes of type 2 diabetic mice.

Authors
Choo, HJ; Kim, JH; Kwon, OB; Lee, CS; Mun, JY; Han, SS; Yoon, YS; Yoon, G; Choi, KM; Ko, YG
Citation
Diabetologia, 49(4):784-791, 2006
Journal Title
Diabetologia
ISSN
0012-186X1432-0428
Abstract
AIMS/HYPOTHESIS: The aim of this study was to confirm a link between mitochondrial dysfunction and type 2 diabetes.



MATERIALS AND METHODS: Cellular levels of mitochondrial proteins, cellular mitochondrial DNA content, and mitochondrial function and morphology were assessed by MitoTracker staining and electron microscopy, in white adipose tissue of 12-week-old male wild-type, obese (ob/ob), and diabetic (db/db) mice.



RESULTS: Levels of mitochondrial proteins were found to be very similar in the livers and muscles of all the mice studied. However, levels were greatly decreased in the adipocytes of db/db mice, but not in those of the wild-type and ob/ob mice. Levels of mitochondrial DNA were also found to be considerably reduced in the adipocytes of db/db mice. MitoTracker staining and under electron microscopy revealed that the number of mitochondria was reduced in adipocytes of db/db mice. Respiration and fatty acid oxidation studies indicated mitochondrial dysfunction in adipocytes of db/db mice. Interestingly, there was an increase in mitochondria and mitochondrial protein production in adipocytes of db/db mice treated with rosiglitazone, an agent that enhances insulin sensitivity.



CONCLUSIONS/INTERPRETATION: Taken together, these data indicate that mitochondrial loss in adipose tissue is correlated with the development of type 2 diabetes.
MeSH terms
Adipocytes/drug effectsAdipocytes/metabolism*Adipocytes/ultrastructureAnimalsDNA, Mitochondrial/geneticsDiabetes Mellitus, Type 2/geneticsDiabetes Mellitus, Type 2/metabolism*Diabetes Mellitus, Type 2/pathologyFemaleMaleMiceMicroscopy, ElectronMitochondria/drug effectsMitochondria/metabolism*Mitochondria/ultrastructureMitochondrial Proteins/biosynthesisMitochondrial Proteins/geneticsRatsThiazolidinediones/pharmacology
DOI
10.1007/s00125-006-0170-2
PMID
16501941
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
AJOU Authors
윤, 계순
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