49 391

Cited 0 times in

Ionizing radiation induces astrocyte gliosis through microglia activation.

Hwang, SY; Jung, JS; Kim, TH; Lim, SJ; Oh, ES; Kim, JY; Ji, KA; Joe, EH; Cho, KH; Han, IO
Neurobiology of disease, 21(3):457-467, 2006
Journal Title
Neurobiology of disease
The aim of this study was to investigate the role of microglia in radiation-induced astrocyte gliosis. We found that a single dose of 15 Gy radiation to a whole rat brain increased immunostaining of glial fibrillary acidic protein in astrocytes 6 h later, and even more so 24 h later, indicating the initiation of gliosis. While irradiation of cultured rat astrocytes had little effect, irradiation of microglia-astrocyte mixed-cultures displayed altered astrocyte phenotype into more processed, which is another characteristic of gliosis. Experiments using microglia-conditioned media indicated this astrocyte change was due to factors released from irradiated microglia. Irradiation of cultured mouse microglial cells induced a dose-dependent increase in mRNA levels for cyclooxygenase-2 (COX-2), interleukin (IL)-1beta, IL-6, IL-18, tumor necrosis factor-alpha and interferon-gamma-inducible protein-10, which are usually associated with microglia activation. Consistent with these findings, irradiation of microglia activated NF-kappaB, a transcription factor that regulates microglial activation. Addition of prostaglandin E2 (PGE2: a metabolic product of the COX-2 enzyme) to primary cultured rat astrocytes resulted in phenotypic changes similar to those observed in mixed-culture experiments. Therefore, it appears that PGE(2) released from irradiated microglia is a key mediator of irradiation-induced gliosis or astrocyte phenotype change. These data suggest that radiation-induced microglial activation and resultant production of PGE2 seems to be associated with an underlying cause of inflammatory complications associated with radiation therapy for malignant gliomas.
MeSH terms
AnimalsAstrocytes/drug effectsAstrocytes/pathologyAstrocytes/radiation effects*Cells, CulturedCoculture TechniquesCulture Media, ConditionedCyclooxygenase 2/drug effectsCyclooxygenase 2/metabolismCyclooxygenase Inhibitors/pharmacologyDinoprostone/metabolismDinoprostone/radiation effectsElectrophoretic Mobility Shift AssayGlial Fibrillary Acidic Protein/metabolismGliosis/etiology*vGliosis/pathologyImmunoblottingImmunohistochemistryMiceMicroglia/drug effectsMicroglia/metabolismMicroglia/radiation effects*Nitrobenzenes/pharmacologyRNA, Messenger/analysisRadiation, IonizingRatsReverse Transcriptase Polymerase Chain ReactionSulfonamides/pharmacology
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
AJOU Authors
조, 은혜
Full Text Link
RIS (EndNote)
XLS (Excel)


해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.