0 297

Cited 0 times in

Ratiometric Detection of gamma-Glutamyltransferase in Human Colon Cancer Tissues Using a Two-Photon Probe

DC Field Value Language
dc.contributor.authorKim, YJ-
dc.contributor.authorPark, SJ-
dc.contributor.authorLim, CS-
dc.contributor.authorLee, DJ-
dc.contributor.authorNoh, CK-
dc.contributor.authorLee, K-
dc.contributor.authorShin, SJ-
dc.contributor.authorKim, HM-
dc.date.accessioned2020-11-17T05:33:00Z-
dc.date.available2020-11-17T05:33:00Z-
dc.date.issued2019-
dc.identifier.issn0003-2700-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/19122-
dc.description.abstractgamma-Glutamyltransferase (GGT) plays a role in cleaving the gamma-glutamyl bond of glutathione. The GGT is known to be overexpressed in some tumors and has been recognized as a potential biomarker for malignant tumors. Colon cancer is one of the most common cancers worldwide: however, there is no quantitative method for detecting cancer cells in human colon tissues. In this study, we report a ratiometric two-photon probe for GGT that can be applied in human colon tissues. The probe (Probe 2) showed high fluorescence efficiency, marked fluorescence changes, excellent kinetics, and selectivity for the GGT in live colon cells. Additionally, we obtained ratiometric two-photon microscopy images of GGT activity in human colon tissue. We used this method to compare normal and cancer tissues based on their ratio values: the ratio value was higher in cancer tissue than in normal tissue. This study provides a method for quantitative analysis of GGT, particularly in human colon cancer, which will be useful for studying GGT-related diseases and diagnosing colon cancer.-
dc.language.isoen-
dc.subject.MESHBiomarkers, Tumor / analysis-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHColonic Neoplasms / diagnostic imaging-
dc.subject.MESHColonic Neoplasms / enzymology-
dc.subject.MESHFluorescent Dyes / chemical synthesis-
dc.subject.MESHFluorescent Dyes / chemistry-
dc.subject.MESHFluorescent Dyes / radiation effects-
dc.subject.MESHGlutamates / chemical synthesis-
dc.subject.MESHGlutamates / chemistry-
dc.subject.MESHGlutamates / radiation effects-
dc.subject.MESHHumans-
dc.subject.MESHMicroscopy, Fluorescence / methods-
dc.subject.MESHNaphthalenes / chemical synthesis-
dc.subject.MESHNaphthalenes / chemistry-
dc.subject.MESHNaphthalenes / radiation effects-
dc.subject.MESHPhotons-
dc.subject.MESHgamma-Glutamyltransferase / analysis-
dc.titleRatiometric Detection of gamma-Glutamyltransferase in Human Colon Cancer Tissues Using a Two-Photon Probe-
dc.typeArticle-
dc.identifier.pmid31265245-
dc.contributor.affiliatedAuthor노, 충균-
dc.contributor.affiliatedAuthor신, 성재-
dc.type.localJournal Papers-
dc.identifier.doi10.1021/acs.analchem.9b02137-
dc.citation.titleAnalytical chemistry-
dc.citation.volume91-
dc.citation.number14-
dc.citation.date2019-
dc.citation.startPage9246-
dc.citation.endPage9250-
dc.identifier.bibliographicCitationAnalytical chemistry, 91(14). : 9246-9250, 2019-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.identifier.eissn1520-6882-
dc.relation.journalidJ000032700-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
Files in This Item:
There are no files associated with this item.

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse