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Discrimination between Human Colorectal Neoplasms with a Dual-Recognitive Two-Photon Probe

Lee, HW; Lim, CS; Choi, H; Cho, MK; Noh, CK; Lee, K; Shin, SJ; Kim, HM
Analytical chemistry, 91(22). : 14705-14711, 2019
Journal Title
Analytical chemistry
Colorectal cancer is a major cause of cancer-related deaths worldwide. Histologic diagnosis using biopsy samples of colorectal neoplasms is the most important step in determining the treatment methods, but these methods have limitations in accuracy and effectiveness. Herein, we report a dual-recognition two-photon probe and its application in the discrimination between human colorectal neoplasms. The probe is composed of two monosaccharides, d-glucosamine and beta-d-galactopyranoside, in a fluorophore for the monitoring of both glucose uptake and beta-gal hydrolysis. In vitro/cell imaging studies revealed the excellent selectivity and sensitivity of the probe for glucose transporter-mediated glucose uptake and beta-gal activity. Cancer-specific uptake was monitored by increased fluorescence intensity, and additional screening of cancer cells was achieved by changes in emission ratio owing to the higher activity of beta-gal. Using human colon tissues and two-photon microscopy, we found that the plot of intensity versus ratio can accurately discriminate between colorectal neoplasms in the order of cancer progression (normal, adenoma, and carcinoma).
MeSH terms
Adenoma / diagnostic imagingCarcinoma / diagnostic imagingCell Line, TumorColorectal Neoplasms / classificationColorectal Neoplasms / diagnostic imaging*Fluorescent Dyes / chemical synthesisFluorescent Dyes / chemistry*Fluorescent Dyes / metabolismFluorescent Dyes / radiation effectsGalactosides / chemical synthesisGalactosides / chemistry*Galactosides / metabolismGalactosides / radiation effectsGlucosamine / analogs & derivatives*Glucosamine / chemical synthesisGlucosamine / metabolismGlucosamine / radiation effectsHumansMicroscopy, Fluorescence / methodsPhotonsbeta-Galactosidase / metabolism
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
AJOU Authors
노, 충균신, 성재
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