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The role of F-actin binding protein Cotl1 in regulation of mitochondrial dynamics
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 정, 선용 | - |
| dc.contributor.author | KANWAL, MEMOONA | - |
| dc.date.accessioned | 2021-01-06T02:34:30Z | - |
| dc.date.available | 2021-01-06T02:34:30Z | - |
| dc.date.issued | 2020 | - |
| dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/19213 | - |
| dc.description.abstract | 비공개 | - |
| dc.description.tableofcontents | I. INTRODUCTION 1
II. MATERIALS AND METHODS 10 A. Annealing control primer (ACP)-based screening of differentially expressed genes 10 B. Plasmids, shRNA constructs and oligonucleotide sequence for siRNA 11 C. Cell culture and drugs treatment 12 D. Antibodies 12 E. Immunofluorescence 13 F. Confocal microscopy 13 G. Transmission electron microscopy 14 H. Primary cell culture 15 I. Quantification of mitochondrial length 16 J. Western blotting 16 K. Cell fractionation 17 L. Statistical analysis 17 III. RESULTS 18 A. Identification of differentially expressed genes among three types of HeLa cells with different mitochondrial morphology 18 B. Cellular localization of endogenous Cotl1 and overexpressed GFP-Cotl1 in HeLa, C2C12, and IMR90 cells 25 C. Changes in Cotl1 distribution in elongated mitochondria in shDrp1 cells 29 D. Changes in mitochondrial and F-actin morphologies in Cotl1-knock-down cells 32 E. Alterations in the expression of mitochondrial-shaping proteins in Cotl1-knockdown cells 37 F. Alterations in mitochondrial morphology and mitochondrial-shaping protein expression in primary cultured heart cells derived from Cotl1-knockout mouse 39 IV. DISCUSSION 42 V. CONCLUSION 46 REFERENCES 47 국문요약 55 | - |
| dc.language.iso | en | - |
| dc.title | The role of F-actin binding protein Cotl1 in regulation of mitochondrial dynamics | - |
| dc.title.alternative | F-actin 결합 Cotl1 단백질의 미토콘드리아 다이나믹스 조절에 관여하는 역할 | - |
| dc.type | Thesis | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000029961 | - |
| dc.subject.keyword | mitochondria | - |
| dc.subject.keyword | mitochondrial dynamics | - |
| dc.subject.keyword | morphology | - |
| dc.subject.keyword | fusion | - |
| dc.subject.keyword | fission | - |
| dc.subject.keyword | mitochondrial-shaping protein | - |
| dc.subject.keyword | Cotl1 (coactosine-like protein 1) | - |
| dc.subject.keyword | F-actin | - |
| dc.subject.keyword | cytoskeleton | - |
| dc.subject.keyword | Drp1 (dynamic related protein 1) | - |
| dc.subject.keyword | gene knockdown | - |
| dc.subject.keyword | gene knockout | - |
| dc.subject.keyword | 미토콘드리아 | - |
| dc.subject.keyword | 미토콘드리아 다이나믹스 | - |
| dc.subject.keyword | 형태 | - |
| dc.subject.keyword | 융합 | - |
| dc.subject.keyword | 분열 | - |
| dc.subject.keyword | 미토콘드리아 형태조절 단백질 | - |
| dc.subject.keyword | Cotl1 (coactosine-like protein 1) | - |
| dc.subject.keyword | F-actin | - |
| dc.subject.keyword | 세포골격 | - |
| dc.subject.keyword | Drp1 (dynamic related protein 1) | - |
| dc.subject.keyword | 유전자억제 | - |
| dc.subject.keyword | 유전자제거 | - |
| dc.description.degree | Master | - |
| dc.contributor.department | 대학원 의생명과학과 | - |
| dc.contributor.affiliatedAuthor | KANWAL, MEMOONA | - |
| dc.date.awarded | 2020 | - |
| dc.type.local | Theses | - |
| dc.citation.date | 2020 | - |
| dc.embargo.liftdate | 9999-12-31 | - |
| dc.embargo.terms | 9999-12-31 | - |
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- Theses > Graduate School of Biomedical Sciences > Master
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