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Characterization of orthotopic xenograft glioblastoma models
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 서, 해영 | - |
| dc.contributor.author | 한, 지훈 | - |
| dc.date.accessioned | 2021-01-06T02:34:31Z | - |
| dc.date.available | 2021-01-06T02:34:31Z | - |
| dc.date.issued | 2020 | - |
| dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/19214 | - |
| dc.description.abstract | 비공개 | - |
| dc.description.tableofcontents | I. INTRODUCTION 1
II. MATERIALS AND METHODS 9 1. Cell culture and transduction 9 2. In vivo xenograft mouse model 10 3. Measurement of tumor volume 10 4. Tissue preparation 11 5. Immunohistochemistry 11 6. Wound healing assay and transwell migration assay 13 7. RT-qPCR 14 8. Western blot 15 9. Cell culture and preparation of cell lines 16 10. In vitro suicide effects 16 11. In vitro bystander effects 17 12. Isobologram analysis 18 13. Statistical Analysis 19 III. RESULTS Part A. Characterization of orthotopic xenograft glioblastoma model 1. Preparation of GFP-expressing LN229 21 2. Compare U87 and LN229 cells in orthotopic xenograft mouse model 21 3. Tumor invasion in an orthotopic animal model 22 4. In vitro assessment of GBM cell migration and invasion 23 5. TME surrounding the tumor mass of U87 and LN229 25 Part B. Combined effects of suicidal gene-expressing mesenchymal stem cells and chemotherapy in experimental glioblastoma model 1. Cytotoxic effect of 5-Fluorouracil and Temozolomide 27 2. Bystander effect of MSC/CD with 5-Flucytosine 27 3. Combination effects of 5-FU with TMZ or MSC/CD with TMZ 28 4. Therapeutic effect of MSC/CD with TMZ in xenograft mouse model 29 IV. DISCUSSION & CONCLUSION 41 V. REFERENCES 45 V. 국문요약 49 | - |
| dc.language.iso | en | - |
| dc.title | Characterization of orthotopic xenograft glioblastoma models | - |
| dc.title.alternative | 교모세포종 이종 이식 모델의 특성 분석 | - |
| dc.type | Thesis | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000029884 | - |
| dc.subject.keyword | Glioblastoma | - |
| dc.subject.keyword | U87 | - |
| dc.subject.keyword | LN229 | - |
| dc.subject.keyword | Temozolomide | - |
| dc.subject.keyword | Mesenchymal stem cells | - |
| dc.subject.keyword | Cytosine deaminase | - |
| dc.subject.keyword | 교모세포종 | - |
| dc.subject.keyword | U87 | - |
| dc.subject.keyword | LN229 | - |
| dc.subject.keyword | 테모졸로마이드 | - |
| dc.subject.keyword | 중간엽 줄기 세포 | - |
| dc.subject.keyword | 사이토신 데 아미나 제 | - |
| dc.description.degree | Master | - |
| dc.contributor.department | 대학원 의생명과학과 | - |
| dc.contributor.affiliatedAuthor | 한, 지훈 | - |
| dc.date.awarded | 2020 | - |
| dc.type.local | Theses | - |
| dc.citation.date | 2020 | - |
| dc.embargo.liftdate | 9999-12-31 | - |
| dc.embargo.terms | 9999-12-31 | - |
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