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Therapeutic effects of intra-arterial MSC-Neurogenin1 transplantation in a rat model of chronic ischemic stroke

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dc.contributor.author조, 다롱-
dc.date.accessioned2021-01-06T02:34:37Z-
dc.date.available2021-01-06T02:34:37Z-
dc.date.issued2020-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/19224-
dc.description.abstract비공개-
dc.description.tableofcontentsⅠ PART 1 1
A. INTRODUCTION 1
B. MATERIALS AND METHODS 2
1. Animals 2
2. Focal ischemic stroke model 2
3. Evaluation of damage to the brain 3
4. Immunohistochemistry of death-related molecules 3
5. Quantification 4
6. Statistical analysis 4
C. RESULTS 5
1. Time-dependent progression of cerebral infarct after ischemic stroke 5
2. Reactive astrocytes and glial scar formation after ischemic stroke 9
3. Inflammatory responses related to activated microglia and macrophage after ischemic stroke 11
4. Apoptotic cell death after ischemic stroke 14
D. DISCUSSION 20
Ⅱ PART 2 23
A. INTRODUCTION 23
B. MATERIALS AND METHODS 25
1. Animals 25
2. Chronic ischemic stroke model and validation 25
3. Human bone marrow-derived MSCs and MSC/Ngn1 preparation 25
4. Intra-arterial mannitol treatment and cell transplantation 26
5. Cell labeling and in vivo tracing 26
6. Magnetic resonance imaging (MRI) 27
7. Immunohistochemistry 27
8. Genomic DNA extraction and quantification 28
9. Behavioral testing 29
10. Statistical analysis 29
C. RESULTS 30
1. Mannitol-induced changes in endothelial tight junction proteins 30
2. In vitro migration assay and cell resistance after hypoxic exposure 33
3. Optimal route and time window of mannitol treatment to BBB permeation in the normal and chronic stroke models 35
4. Stem cell delivery via IA in increased with BBB breakage/mannitol 39
5. Distribution and extravasation of intra-arterially administered cells after mannitol infusion 41
6. Neural differentiation of MSC/Ngn1 in a rat chronic stroke model 44
7. Evaluation the therapeutic effects of MSC/Ngn1 47
D. DISCUSSIONS 49
E. REFERENCES 51		-
dc.language.isoen-
dc.titleTherapeutic effects of intra-arterial MSC-Neurogenin1 transplantation in a rat model of chronic ischemic stroke-
dc.title.alternative만성 허혈성 뇌졸중 동물 모델에서 MSC-Neurogenin1 동맥내 이식의 치료적 효능에 관한 연구-
dc.typeThesis-
dc.identifier.urlhttp://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000030330-
dc.subject.keywordBrain ischemia-
dc.subject.keywordmesenchymal stem cell-
dc.subject.keywordchronic stroke-
dc.subject.keywordmannitol-
dc.subject.keywordintra-arterial transplantation-
dc.subject.keyword뇌경색-
dc.subject.keyword중간엽 줄기세포-
dc.subject.keyword만성 뇌졸중-
dc.subject.keyword만니톨-
dc.subject.keyword동맥내 이식-
dc.description.degreeDoctor-
dc.contributor.department대학원 의생명과학과-
dc.contributor.affiliatedAuthor조, 다롱-
dc.date.awarded2020-
dc.type.localTheses-
dc.citation.date2020-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
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