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An intracellular antibody can suppress tumorigenicity in hepatitis B virus X-expressing cells.

Authors
Jin, YH; Kwon, MH; Kim, K; Shin, HJ; Shin, JS; Cho, H; Park, S
Citation
Cancer immunology, immunotherapy : CII, 55(5):569-578, 2006
Journal Title
Cancer immunology, immunotherapy : CII
ISSN
0340-70041432-0851
Abstract
Although the hepatitis B virus X protein (HBx) is thought to play a causative role in the development of hepatocellular carcinoma, it is not yet known whether interfering with HBx function may affect the cellular transformation of HBx-expressing tumor cells. To address this question, we adopted an intracellular antibody fragment expression approach to block the function of HBx. Expression of a single-chain variable fragment (scFv) specific to HBx (designated as H7scFv) inhibited HBx-dependent cellular transactivation. Furthermore, H7scFv suppressed the growth of HBx-expressing tumor cells in both soft agar and nude mice. The suppressive effect of H7scFv on tumorigenicity appeared not to be mediated by inhibition of HBx-induced growth stimulation since the growth rate of these cells was not affected significantly by H7scFv expression. In conclusion, these data suggest that the HBx-dependent transformed phenotype is reversible and that HBx may be a good molecular target for the treatment of HBV-related tumors.
MeSH terms
AnimalsAntibodies, Monoclonal/immunologyBlotting, WesternCarcinoma, Hepatocellular/metabolism*Carcinoma, Hepatocellular/virologyCell Transformation, Neoplastic/metabolism*Cytoplasm/immunologyFluorescent Antibody TechniqueImmunoglobulin Fragments/immunology*ImmunoprecipitationLiver Neoplasms, Experimental/immunologyLiver Neoplasms, Experimental/metabolismLiver Neoplasms, Experimental/virologyMiceNIH 3T3 CellsReverse Transcriptase Polymerase Chain ReactionTrans-Activators/immunologyTrans-Activators/metabolism*Transcriptional Activation
DOI
10.1007/s00262-005-0037-2
PMID
16273352
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
AJOU Authors
권, 명희김, 경민신, 호준조, 혜성박, 선
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