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Intracellular antibody fragment against hepatitis B virus X protein does not inhibit viral replication.

Authors
Jin, YH; Hong, SH; Kim, K; Shin, HJ; Park, S
Citation
Yonsei medical journal, 47(5):721-728, 2006
Journal Title
Yonsei medical journal
ISSN
0513-57961976-2437
Abstract
Replication of the hepatitis B virus is suppressed by deficiency of the X protein. Although several molecules that block cellular targets of X protein reduce the production of hepatitis B virus progeny, the effect of a specific inhibitor of X protein on viral replication has not been investigated. To block X protein specifically, we adopted an intracellular expression approach using H7 single chain variable fragment (H7scFv), an antibody fragment against X protein. We previously demonstrated that cytoplasmic expression of H7scFv inhibits X protein-induced tumorigenicity and transactivation. In this study, intracellular H7scFv expression inhibits reporter gene transactivation but not viral replication determined by endogenous hepatitis B virus polymerase activity assay and real-time PCR. Our findings imply that intracellular expression of antibody fragment against X protein may not be an alternative therapeutic modality for inhibition of hepatitis B virus replication.
MeSH terms
Cell LineHepatitis B e Antigens/metabolismHepatitis B virus/drug effects*Hepatitis B virus/physiologyImmunoglobulin Variable Region/geneticsImmunoglobulin Variable Region/metabolismImmunoglobulin Variable Region/pharmacology*Trans-Activators/antagonists & inhibitors*Trans-Activators/immunologyVirus Replication/drug effects*
DOI
10.3349/ymj.2006.47.5.721
PMID
17066517
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
홍, 승호김, 경민신, 호준박, 선
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