Cited 23 times in

Mitochondria ubiquitin ligase, MARCH5 resolves hepatitis B virus X protein aggregates in the liver pathogenesis

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dc.contributor.authorYoo, YS-
dc.contributor.authorPark, YJ-
dc.contributor.authorLee, HS-
dc.contributor.authorOanh, NTK-
dc.contributor.authorCho, MY-
dc.contributor.authorHeo, J-
dc.contributor.authorLee, ES-
dc.contributor.authorCho, H-
dc.contributor.authorPark, YY-
dc.contributor.authorCho, H-
dc.date.accessioned2022-01-14T05:18:58Z-
dc.date.available2022-01-14T05:18:58Z-
dc.date.issued2019-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/20090-
dc.description.abstractInfection of hepatitis B virus (HBV) increase the incidence of chronic liver disease and hepatocellular carcinoma (HCC). The hepatitis B viral x (HBx) protein encoded by the HBV genome contributes to the pathogenesis of HCC and thus, negative regulation of HBx is beneficial for the alleviation of the disease pathogenesis. MARCH5 is a mitochondrial E3 ubiquitin ligase and here, we show that high MARCH5 expression levels are correlated with improved survival in HCC patients. MARCH5 interacts with HBx protein mainly accumulated in mitochondria and targets it for degradation. The N-terminal RING domain of MARCH5 was required for the interaction with HBx, and MARCH5(H43W) lacking E3 ligase activity failed to reduce HBx protein levels. High expression of HBx results in the formation of protein aggregates in semi-denaturing detergent agarose gels and MARCH5 mediates the elimination of protein aggregates through the proteasome pathway. HBx-induced ROS production, mitophagy, and cyclooxygenase-2 gene expression were suppressed in the presence of high MARCH5 expression. These results suggest MARCH5 as a target for alleviating HBV-mediated liver disease.-
dc.subject.MESHCarcinoma, Hepatocellular / etiology-
dc.subject.MESHCarcinoma, Hepatocellular / metabolism-
dc.subject.MESHCarcinoma, Hepatocellular / mortality-
dc.subject.MESHCarcinoma, Hepatocellular / pathology-
dc.subject.MESHHEK293 Cells-
dc.subject.MESHHeLa Cells-
dc.subject.MESHHepatitis B / complications-
dc.subject.MESHHepatitis B / metabolism-
dc.subject.MESHHepatitis B / virology-
dc.subject.MESHHepatitis B virus / chemistry-
dc.subject.MESHHumans-
dc.subject.MESHLiver Neoplasms / etiology-
dc.subject.MESHLiver Neoplasms / metabolism-
dc.subject.MESHLiver Neoplasms / mortality-
dc.subject.MESHLiver Neoplasms / pathology-
dc.subject.MESHMembrane Proteins / genetics-
dc.subject.MESHMembrane Proteins / metabolism-
dc.subject.MESHMitochondria / metabolism-
dc.subject.MESHProtein Aggregates-
dc.subject.MESHProtein Aggregation, Pathological / metabolism-
dc.subject.MESHProteolysis-
dc.subject.MESHSurvival Rate-
dc.subject.MESHTrans-Activators / metabolism-
dc.subject.MESHTransfection-
dc.subject.MESHUbiquitin-Protein Ligases / genetics-
dc.subject.MESHUbiquitin-Protein Ligases / metabolism-
dc.subject.MESHViral Regulatory and Accessory Proteins / metabolism-
dc.titleMitochondria ubiquitin ligase, MARCH5 resolves hepatitis B virus X protein aggregates in the liver pathogenesis-
dc.typeArticle-
dc.identifier.pmid31819032-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901512/-
dc.contributor.affiliatedAuthorLee, HS-
dc.contributor.affiliatedAuthorOanh, NTK-
dc.contributor.affiliatedAuthorCho, MY-
dc.contributor.affiliatedAuthorCho, H-
dc.type.localJournal Papers-
dc.identifier.doi10.1038/s41419-019-2175-z-
dc.citation.titleCell death & disease-
dc.citation.volume10-
dc.citation.number12-
dc.citation.date2019-
dc.citation.startPage938-
dc.citation.endPage938-
dc.identifier.bibliographicCitationCell death & disease, 10(12). : 938-938, 2019-
dc.identifier.eissn2041-4889-
dc.relation.journalidJ020414889-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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