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Receptor mediation and nociceptin inhibition of bradykinin-induced plasma extravasation in the knee joint of the rat.

Moriyama, K; Liu, J; Jang, Y; Chae, YJ; Wang, Y; Mitchell, J; Grond, S; Han, X; Xing, Y; Xie, GX; Pierce Palmer, P
Inflammation research : official journal of the European Histamine Research Society, 58(12):873-880, 2009
Journal Title
Inflammation research : official journal of the European Histamine Research Society
OBJECTIVE AND DESIGN: The aim was to investigate the signaling mechanisms and regulation of bradykinin (BK)-induced inflammation in rat knee joint.

MATERIALS AND METHODS: Knee joints of anesthetized rats were perfused with BK (0.1-1.0 microM), and synovial plasma extravasation (PE) was evaluated by spectrophotometrical measurement of Evans Blue leakage. To examine the signaling pathway, B1 antagonist [des-Arg10]-HOE140 (0.1-1.0 microM) and B2 antagonist HOE140 (0.05-1.0 microM), calcitonin gene-related peptide (CGRP) antagonist CGRP8-37 (0.5-1.0 microM), prostaglandin E2 antagonist AH-6809 (0.1-1.0 microM), and histamine H1 antagonist mepyramine (0.1-1.0 microM) were used. Nociceptin (0.0001-1.0 microM) and antagonist J-113397 were tested for modulation of BK-induced PE. The analyses were compared side-by-side with 5-hydroxytryptamine-induced PE.

RESULTS: BK perfusion dose-dependently induced PE, which was blocked by HOE140, CGRP8-37, AH-6809, and mepyramine. It was also inhibited by nociceptin, which could be reversed by antagonist J-113397. In contrast, 5-hydroxytryptamine-induced PE was biphasically regulated by nociceptin and was not antagonized by CGRP8-37.

CONCLUSIONS: BK-induced PE is mediated by B2 receptors and may involve CGRP, prostaglandin, and histamine pathways. BK-induced PE is inhibited by nociceptin through the activation of ORL1 receptors. There are differences between BK- and 5-hydroxytryptamine-induced inflammation in signaling and modulation.
MeSH terms
AnimalsBradykinin/metabolism*Calcitonin Gene-Related Peptide/metabolismColoring Agents/metabolismDinoprostone/antagonists & inhibitorsDinoprostone/metabolismEvans Blue/metabolismHistamine/metabolismHumansKnee Joint/metabolism*MaleOpioid Peptides/metabolism*Plasma/metabolism*RatsRats, Sprague-DawleyReceptor, Bradykinin B1/antagonists & inhibitorsReceptor, Bradykinin B1/metabolism*Receptor, Bradykinin B2/antagonists & inhibitorsReceptor, Bradykinin B2/metabolism*Serotonin/metabolismSignal Transduction/physiology
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Journal Papers > School of Medicine / Graduate School of Medicine > Anesthesiology & Pain Medicine
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채, 윤정
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