74 283

Cited 2 times in

Receptor mediation and nociceptin inhibition of bradykinin-induced plasma extravasation in the knee joint of the rat.

Authors
Moriyama, K; Liu, J; Jang, Y; Chae, YJ; Wang, Y; Mitchell, J; Grond, S; Han, X; Xing, Y; Xie, GX; Pierce Palmer, P
Citation
Inflammation research : official journal of the European Histamine Research Society, 58(12):873-880, 2009
Journal Title
Inflammation research : official journal of the European Histamine Research Society
ISSN
1023-38301420-908X
Abstract
OBJECTIVE AND DESIGN: The aim was to investigate the signaling mechanisms and regulation of bradykinin (BK)-induced inflammation in rat knee joint. MATERIALS AND METHODS: Knee joints of anesthetized rats were perfused with BK (0.1-1.0 microM), and synovial plasma extravasation (PE) was evaluated by spectrophotometrical measurement of Evans Blue leakage. To examine the signaling pathway, B1 antagonist [des-Arg10]-HOE140 (0.1-1.0 microM) and B2 antagonist HOE140 (0.05-1.0 microM), calcitonin gene-related peptide (CGRP) antagonist CGRP8-37 (0.5-1.0 microM), prostaglandin E2 antagonist AH-6809 (0.1-1.0 microM), and histamine H1 antagonist mepyramine (0.1-1.0 microM) were used. Nociceptin (0.0001-1.0 microM) and antagonist J-113397 were tested for modulation of BK-induced PE. The analyses were compared side-by-side with 5-hydroxytryptamine-induced PE. RESULTS: BK perfusion dose-dependently induced PE, which was blocked by HOE140, CGRP8-37, AH-6809, and mepyramine. It was also inhibited by nociceptin, which could be reversed by antagonist J-113397. In contrast, 5-hydroxytryptamine-induced PE was biphasically regulated by nociceptin and was not antagonized by CGRP8-37. CONCLUSIONS: BK-induced PE is mediated by B2 receptors and may involve CGRP, prostaglandin, and histamine pathways. BK-induced PE is inhibited by nociceptin through the activation of ORL1 receptors. There are differences between BK- and 5-hydroxytryptamine-induced inflammation in signaling and modulation.
MeSH terms
AnimalsBradykinin/metabolism*Calcitonin Gene-Related Peptide/metabolismColoring Agents/metabolismDinoprostone/antagonists & inhibitorsDinoprostone/metabolismEvans Blue/metabolismHistamine/metabolismHumansKnee Joint/metabolism*MaleOpioid Peptides/metabolism*Plasma/metabolism*RatsRats, Sprague-DawleyReceptor, Bradykinin B1/antagonists & inhibitorsReceptor, Bradykinin B1/metabolism*Receptor, Bradykinin B2/antagonists & inhibitorsReceptor, Bradykinin B2/metabolism*Serotonin/metabolismSignal Transduction/physiology
DOI
10.1007/s00011-009-0058-y
PMID
19544046
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anesthesiology & Pain Medicine
AJOU Authors
채윤정
Full Text Link
Files in This Item:
873-880.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse