32 289

Cited 0 times in

Effects of low-intensity ultrasound (LIUS) stimulation on human cartilage explants.

Min, BH; Woo, JI; Cho, HS; Choi, BH; Park, SJ; Choi, MJ; Park, SR
Scandinavian journal of rheumatology, 35(4):305-311, 2006
Journal Title
Scandinavian journal of rheumatology
OBJECTIVE: To evaluate the effects of low-intensity ultrasound (LIUS) stimulation on the anabolic state of human cartilage from patients with osteoarthritis (OA).

METHODS: Explant cultures of human OA cartilage were stimulated for 10 min every day for 7 consecutive days using continuous-wave sonication at a frequency of 1 MHz with spatial and temporal average intensities of 0 (control), 40, 200, 500, or 700 mW/cm2. The effects of LIUS on cell proliferation were evaluated by 3H-thymidine incorporation. Proteoglycan synthesis was evaluated by the incorporation of 35S-sulfate and by Safaranin O staining. Collagen synthesis was evaluated by 3H-proline incorporation and immunohistochemistry.

RESULTS: At an intensity of 200 mW/cm2, LIUS treatment induced the expression of collagen type II and proteoglycan measured by the incorporation of radioactivity and specific staining of the cartilage explants. However, the expression decreased again at the higher intensities of 500 or 700 mW/cm2. Ultrasound had no stimulatory effect on cell proliferation at any intensity.

CONCLUSION: LIUS has anabolic effects on human cartilage in explant cultures, indicating a potentially important method for the repair of osteoarthritic cartilage.
MeSH terms
AgedCartilage, Articular/growth & developmentCartilage, Articular/metabolismCartilage, Articular/ultrasonography*Cell ProliferationCollagen Type II/biosynthesisFemaleHumansMaleMiddle AgedOsteoarthritis/metabolismProteoglycans/biosynthesisTissue Culture TechniquesUltrasonography/methods
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
AJOU Authors
민, 병현
Full Text Link
RIS (EndNote)
XLS (Excel)


해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.