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An electromagnetic compressive force by cell exciter stimulates chondrogenic differentiation of bone marrow-derived mesenchymal stem cells.

Authors
Park, SH; Sim, WY; Park, SW; Yang, SS; Choi, BH; Park, SR; Park, K; Min, BH
Citation
Tissue engineering, 12(11):3107-3117, 2006
Journal Title
Tissue engineering
ISSN
1076-32791557-8690
Abstract
In this study, we present a biological micro-electromechanical system and its application to the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells (MSCs). Actuated by an electromagnetic force, the micro cell exciter was designed to deliver a cyclic compressive load (CCL) with various magnitudes. Two major parts in the system are an actuator and a cartridge-type chamber. The former has a permanent magnet and coil, and the latter is equipped with 7 sample dishes and 7 metal caps. Mixed with a 2.4% alginate solution, the alginate/MSC layers were positioned in the sample dishes; the caps contained chondrogenic defined medium without transforming growth factor-beta (TGF-beta). Once powered, the actuator coil-derived electromagnetic force pulled the metal caps down, compressing the samples. The cyclic load was given at 1-Hz frequency for 10 min twice a day. Samples in the dishes without a cap served as a control. The samples were analyzed at 3, 5, and 7 days after stimulation for cell viability, biochemical assays, histologic features, immunohistochemistry, and gene expression of the chondrogenic markers. Applied to the alginate/MSC layer, the CCL system enhanced the synthesis of cartilage-specific matrix proteins and the chondrogenic markers, such as aggrecan, type II collagen, and Sox9. We found that the micromechanically exerted CCL by the cell exciter was very effective in enhancing the chondrogenic differentiation of MSCs, even without using exogenous TGF-beta.
MeSH terms
Aggrecans/biosynthesisAlginates/pharmacologyAnimalsBiological Markers/metabolismBone Marrow Cells/cytology*Cell DifferentiationCell Survival/drug effectsChondrocytes/cytology*Chondrocytes/physiologyChondrogenesis/physiology*Collagen Type II/biosynthesisCulture Media/chemistryElectromagnetic Phenomena*Equipment DesignFemur/cytologyGene Expression/drug effectsGlucuronic Acid/pharmacologyHexuronic Acids/pharmacologyHigh Mobility Group Proteins/biosynthesisHigh Mobility Group Proteins/geneticsImmunohistochemistryMesenchymal Stem Cells/cytology*RabbitsSOX9 Transcription FactorSolutions/pharmacologyTibia/cytologyTime FactorsTranscription Factors/biosynthesisTranscription Factors/genetics
DOI
10.1089/ten.2006.12.3107
PMID
17518626
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
Journal Papers > Research Organization > Cell Therapy Center
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