One of major problem with cancer chemotherapy is cancer cell's resistance against anticancer drugs. It can be either innate or acquired during chemotherapy. The cancer drug resistance limits choice of anticancer drug and leads to bad prognosis of patients. With advanced NGS technology and RNA sequencing technique, recent studies suggest alternative splicing event as important factor in cancer drug resistance. In our study, we focus on spliceosome and it's alternative splicing modulation in drug resistance of cancer cells. Fluorouracil (5-FU) resistant cancer cell line T98FR was developed by treating T98G cell with moderate dose of 5-FU over passages. T98FR cell had highly up-regulated spliceosome related genes compared to T98G cell. In in-vitro study, T98FR cell became sensitive to 5-FU when cells were treated with Pladienolide-β, a spliceosome inhibitor. In addition, T98FR cell expressed less 5-FU metabolism genes when they were treated with Pladienolide-β
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
Total Visit :10,643,300
Total Download :4,380,706
Today View :149
Ajou University Medical Information & Media Center 164 Worldcup-ro Yeongtong-gu Suwon 16499 Korea / TEL : 031-219-5312 Copyright (c) Ajou University Medical Information & Media Center All Rights Reserved. AJOU Open Repository는 국립중앙도서관 OAK 보급사업으로 구축되었습니다.