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The discovery of LM9 as an SLE specific biomarker using sera of patients with Systemic Lupus Erythematosus(SLE)

Authors
Lee, Sung Min | Lee, Sang Won | Son, In Ok | Baek, Wook Young | Chung, Jee Min | Kang, Ho Chul | Suh, Chang Hee
Department
Department of Rheumatology, Ajou University School of Medicine  | Department of Physiology, Ajou University School of Medicine
Abstract
Systemic lupus erythematosus (SLE) is chronic inflammatory disease caused by production of various autoantibodies such as antinuclear antibodies and anti-dsDNA due to genetic, hormones, and environmental factors. SLE occur frequently in 20-40 year-old women with the ratio of male and female is 1:10. Previous studies have shown that genetic and environmental factors influence onset, but the exact cause of SLE is not understood. SLE diagnoses with various clinical symptoms. For the clearer diagnosis of SLE, patient serum with SLE (n=10) was compared with the normal controls (NCs, n=5) using the 21K protein chip analysis method. As a result, we found 63 lupus specific autoantibodies. Interestingly, LM9 antibody was expressed high in patients with SLE than NCs. We made the GST-LM9 protein to confirm that LM9 antibody is specifically expressed in patient with SLE. We confirmed Dot blot analysis using the sera from NCs (n=50) and patients with SLE (n=100), rheumatoid arthritis (n=25), systemic sclerosis (n=30), and Bechet’s disease (n=15). As a result, it was confirmed that LM9 antibody was detected in 79 out of 100 patients with SLE and it was highly expressed compared to NCs and other autoimmune diseases. The sensitivity of LM9 antibody in SLE patients was 79%, and the specificity was 90%. Therefore, LM9 antibody is the potential to be a specific biomarker of SLE and is expected to be a new biomarker for the diagnosis of SLE.
Keywords
Appears in Collections:
Poster > School of Medicine / Graduate School of Medicine > Department of Rheumatology
Poster > School of Medicine / Graduate School of Medicine > Department of Physiology
Ajou Authors
대학, 류마티스내과학교실  |  대학, 생리학교실
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