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Type I PIP5Kα negatively regulates NGF-induced neurite outgrowth in PC12 cells
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Liu, Tian | - |
| dc.date.accessioned | 2011-04-13T04:48:28Z | - |
| dc.date.available | 2011-04-13T04:48:28Z | - |
| dc.date.issued | 2010 | - |
| dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/2243 | - |
| dc.description.abstract | "Type I phosphatidylinositol 4-phosphate 5-kinase (PIP5KI) is responsible for the production of a membrane lipid, phosphatidylinositol 4,5-bisphosphate (PtdIns[4,5]P2). Here, I have examined whether PIP5KIα, an isoform of PIP5Kα, plays a role in neurite outgrowth. For this, I generated a stable PC12 cell line lacking PIP5KIa by microRNA (miRNA) expression, leading to decreased PtdIns(4,5)P2 level. Results show that neurite outgrowth induced by nerve growth factor (NGF) was more prominent in PIP5KIa-deficient cells than in control miRNA-expressing cells. Reintroduction of PIP5KIα into the PIP5KIα-deficient cells attenuated NGF-induced neurite outgrowth. Similar to the neurite changes, PIP5KIα deficiency promoted NGF-induced Akt phosphorylation that was reversed by the PIP5KIα reintroduction. Treatment of exogenous PtdIns(4,5)P2 also inhibited Akt phosphorylation by NGF. Together, these results suggest that PIP5KIα-driven PtdIns[4,5]P2 acts as a negative regulator of neurite outgrowth by modulating Akt activity.
Key words: PIP5KIα, PtdIns(4,5)P2, NGF, AKT, neurite outgrowth, PC12 " | - |
| dc.description.tableofcontents | "TABLE OF CONTENTS
ABSTRACT =ⅰ TABLE OF CONTENTS =ⅱ LIST OF FIGURES =ⅳ LIST OF ABBREVIATION = Ⅴ I. INTRODUCTION = 1 A. PtdIns(4,5)P2 = 1 B. PIP5Ks = 2 C. Nerve Growth Factor = 2 D. Neurite Outgrowth = 3 E. PC12 cells = 4 F. Aim of this study = 4 II. MATERIAL AND METHOD = 6 A. Materials = 6 B. Methods = 6 1. Cell culture = 6 2. Establishment of stable cell lines = 6 3. PIP5KIα transfection and imaging = 7 4. RT-PCR = 7 5. Western blotting analysis = 7 6. PtdIns(4,5)P2 delivery = 8 7. Immunofluorescence staining = 8 III. RESULTS = 9 A. Establishment of PIP5KIα knockdown and control knockdown cell lines = 9 B. Knocking down the gene of PIP5KIα induces the extension of neurite outgrowth in PC12 cells = 9 C. Knocking down the gene of PIP5KIα induces the activation of AKT and ERK = 9 D. Overexpression of PIP5KIα leads to the retraction of neurite outgrowth and reduction of activation of AKT but not ERK = 13 E. PIP5KIα knockdown decreases PtdIns(4,5)P2 level at the cell surface = 16 F. Delivery of PtdIns(4,5)P2 into PIP5KIα knockdown cells inhibites NGF-induced AKT activation but not ERK activation = 16 G. NGF treatment leads to the reduction of PtdIns (4,5)P2 in plasma membrane = 16 IV. DISCUSSION = 21 V. CONCLUSION = 23 REFERENCES = 24 국문요약 = 30" | - |
| dc.format | application/pdf | - |
| dc.language.iso | en | - |
| dc.title | Type I PIP5Kα negatively regulates NGF-induced neurite outgrowth in PC12 cells | - |
| dc.title.alternative | PC12 세포에서 NGF에 의한 neurite outgrowth에 미치는 PIP5KIa의 저해 작용 | - |
| dc.type | Thesis | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000010857 | - |
| dc.description.degree | Master | - |
| dc.contributor.department | 대학원 의생명과학과 | - |
| dc.contributor.affiliatedAuthor | Liu, Tian | - |
| dc.date.awarded | 2010 | - |
| dc.type.local | Theses | - |
| dc.citation.date | 2010 | - |
| dc.embargo.liftdate | 9999-12-31 | - |
| dc.embargo.terms | 9999-12-31 | - |
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