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Sphere-Forming Culture for Expanding Genetically Distinct Patient-Derived Glioma Stem Cells by Cellular Growth Rate Screening

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dc.contributor.authorShin, K-
dc.contributor.authorShin, H-
dc.contributor.authorCho, HJ-
dc.contributor.authorKang, H-
dc.contributor.authorLee, JK-
dc.contributor.authorSeo, YJ-
dc.contributor.authorShin, YJ-
dc.contributor.authorKim, D-
dc.contributor.authorKoo, H-
dc.contributor.authorKong, DS-
dc.contributor.authorSeol, HJ-
dc.contributor.authorLee, JI-
dc.contributor.authorLee, HW-
dc.contributor.authorNam, DH-
dc.date.accessioned2022-11-29T01:43:16Z-
dc.date.available2022-11-29T01:43:16Z-
dc.date.issued2020-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/22948-
dc.description.abstractDiffusely infiltrating gliomas (DIGs) are difficult to completely resect and are associated with a high rate of tumor relapse and progression from low- to high-grade glioma. In particular, optimized short-term culture-enriching patient-derived glioma stem cells (GSCs) are essential for customizing the therapeutic strategy based on clinically feasible in vitro drug screening for a wide range of DIGs, owing to the high inter-tumoral heterogeneity. Herein, we constructed a novel high-throughput culture condition screening platform called 'GFSCAN', which evaluated the cellular growth rates of GSCs for each DIG sample in 132 serum-free combinations, using 13 previously reported growth factors closely associated with glioma aggressiveness. In total, 72 patient-derived GSCs with available genomic profiles were tested in GFSCAN to explore the association between cellular growth rates in specific growth factor combinations and genomic/molecular backgrounds, including isocitrate dehydrogenase 1 (IDH1) mutation, chromosome arm 1p and 19q co-deletion, ATRX chromatin remodeler alteration, and transcriptional subtype. GSCs were clustered according to the dependency on epidermal growth factor and basic fibroblast growth factor (E&F), and isocitrate dehydrogenase 1 (IDH1) wild-type GSCs showed higher E&F dependencies than IDH1 mutant GSCs. More importantly, we elucidated optimal combinations for IDH1 mutant glioblastoma and lower grade glioma GSCs with low dependencies on E&F, which could be an aid in clinical decision-making for these DIGs. Thus, we demonstrated the utility of GFSCAN in personalizing in vitro cultivation to nominate personalized therapeutic options, in a clinically relevant time frame, for individual DIG patients, where standard clinical options have been exhausted.-
dc.language.isoen-
dc.titleSphere-Forming Culture for Expanding Genetically Distinct Patient-Derived Glioma Stem Cells by Cellular Growth Rate Screening-
dc.typeArticle-
dc.identifier.pmid32120790-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139415-
dc.subject.keyworddiffuse infiltrating glioma-
dc.subject.keywordpersonalized medicine-
dc.subject.keywordglioma stem cells-
dc.subject.keywordshort-term cultivation screening platform-
dc.subject.keywordgrowth factor-
dc.subject.keywordgenomic profiling-
dc.contributor.affiliatedAuthorLee, JK-
dc.type.localJournal Papers-
dc.identifier.doi10.3390/cancers12030549-
dc.citation.titleCancers-
dc.citation.volume12-
dc.citation.number3-
dc.citation.date2020-
dc.citation.startPage549-
dc.citation.endPage549-
dc.identifier.bibliographicCitationCancers, 12(3). : 549-549, 2020-
dc.identifier.eissn2072-6694-
dc.relation.journalidJ020726694-
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Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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