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Molecular characterization of Enterococcus faecium isolated from hospitalized patients in Korea.

Authors
Lee, WG; Lee, SM; Kim, YS
Citation
Letters in applied microbiology, 43(3):274-279, 2006
Journal Title
Letters in applied microbiology
ISSN
0266-82541472-765X
Abstract
AIMS: Multilocus sequence typing (MLST) was performed for vancomycin-resistant Enterococcus faecium (VREF) from diverse geographical areas in Korea to obtain insights into the genetic relationships with other molecular profiles. To understand the diversity of lineages, vancomycin-susceptible E. faecium (VSEF) were included. METHODS AND RESULTS: A total of 60 E. faecium isolates were analysed by MLST and esp profile. Molecular typing of Tn1546 of 30 VREF strains was evaluated by overlapping PCR of Tn1546 and DNA sequencing. Seven sequence types (ST) were found among 30 VSEF isolates, and four STs were found among 30 VREF isolates. The types most frequently encountered were ST 78 (26 isolates) and ST 203 (16 isolates). Of the 60 E. faecium isolates, 35 isolates were positive for the esp gene. On molecular typing of Tn1546, all VREF isolates were divided into four main types. Strains with the same ST showed divergence in Tn1546 types and strains with the same Tn1546 type represented different STs. CONCLUSIONS: An association between Tn1546 typing and MLST was not found. SIGNIFICANCE AND IMPACT OF THE STUDY: These results suggest that the horizontal spread of Tn1546 between strains plays a major role in the dissemination of vancomycin resistance in Korea.
MeSH terms
Bacterial Typing Techniques*DNA Transposable Elements/geneticsEnterococcus faecium/classification*Enterococcus faecium/genetics*Enterococcus faecium/isolation & purificationHospitalsHumansKoreaMicrobial Sensitivity TestsPhylogenyPhysical Chromosome MappingSequence Analysis, DNA*Vancomycin Resistance/genetics*
DOI
10.1111/j.1472-765X.2006.01957.x
PMID
16910931
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Laboratory Medicine
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