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Fabrication of decellularized meniscus extracellular matrix according to inner cartilaginous, middle transitional, and outer fibrous zones result in zone-specific protein expression useful for precise replication of meniscus zones

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dc.contributor.authorYun, HW-
dc.contributor.authorSong, BR-
dc.contributor.authorShin, DI-
dc.contributor.authorYin, XY-
dc.contributor.authorTruong, MD-
dc.contributor.authorNoh, S-
dc.contributor.authorJin, YJ-
dc.contributor.authorKwon, HJ-
dc.contributor.authorMin, BH-
dc.contributor.authorPark, DY-
dc.date.accessioned2023-01-10T00:39:14Z-
dc.date.available2023-01-10T00:39:14Z-
dc.date.issued2021-
dc.identifier.issn0928-4931-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/23917-
dc.description.abstractMeniscus is a fibrocartilage composite tissue with three different microstructual zones, inner fibrocartilage, middle transitional, and outer fibrous zone. We hypothesized that decellularized meniscus extracellular matrix (DMECM) would have different characteristics according to zone of origin. We aimed to compare zone-specific DMECM in terms of biochemical characteristics and cellular interactions associated with tissue engineering. Micronized DMECM was fabricated from porcine meniscus divided into three microstructural zones. Characterization of DMECM was done by biochemical and proteomic analysis. Inner DMECM showed the highest glycosaminoglycan content, while middle DMECM showed the highest collagen content among groups. Proteomic analysis showed significant differences among DMECM groups. Inner DMECM showed better adhesion and migration potential to meniscus cells compared to other groups. DMECM resulted in expression of zone-specific differentiation markers when co-cultured with synovial mesenchymal stem cells (SMSCs). SMSCs combined with inner DMECM showed the highest glycosaminoglycan in vivo. Outer DMECM constructs, on the other hand, showed more fibrous tissue features, while middle DMECM constructs showed both inner and outer zone characteristics. In conclusion, DMECM showed different characteristics according to microstructural zones, and such material may be useful for zone-specific tissue engineering of meniscus.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHExtracellular Matrix-
dc.subject.MESHMenisci, Tibial-
dc.subject.MESHMeniscus-
dc.subject.MESHProteomics-
dc.subject.MESHSwine-
dc.subject.MESHTissue Engineering-
dc.titleFabrication of decellularized meniscus extracellular matrix according to inner cartilaginous, middle transitional, and outer fibrous zones result in zone-specific protein expression useful for precise replication of meniscus zones-
dc.typeArticle-
dc.identifier.pmid34474863-
dc.identifier.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0928-4931(21)00451-3-
dc.subject.keywordDecellularization-
dc.subject.keywordExtracellular matrix-
dc.subject.keywordFibrocartilage-
dc.subject.keywordMeniscus-
dc.subject.keywordTissue engineering.-
dc.contributor.affiliatedAuthorMin, BH-
dc.contributor.affiliatedAuthorPark, DY-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.msec.2021.112312-
dc.citation.titleMaterials science & engineering. C, Materials for biological applications-
dc.citation.volume128-
dc.citation.date2021-
dc.citation.startPage112312-
dc.citation.endPage112312-
dc.identifier.bibliographicCitationMaterials science & engineering. C, Materials for biological applications, 128. : 112312-112312, 2021-
dc.identifier.eissn1873-0191-
dc.relation.journalidJ009284931-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
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