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G-CSF protects human cerebral hybrid neurons against in vitro ischemia.

Authors
Jung, KH; Chu, K; Lee, ST; Kang, L; Kim, SU; Kim, M; Roh, JK
Citation
Neuroscience letters, 394(3):168-173, 2006
Journal Title
Neuroscience letters
ISSN
0304-39401872-7972
Abstract
Granulocyte colony-stimulating factor (G-CSF) protects neurons against experimental focal cerebral ischemia. However, its neuroprotective effect on human brain is unknown. We sought to determine whether G-CSF can protect the human cerebral neurons in vitro. Human cerebral-neuroblastoma hybrid cell line (A1) was exposed to oxygen and glucose deprivation with or without G-CSF. G-CSF promoted cell survival and decreased cytotoxicity effectively at 25 ng/ml. G-CSF reduced early apoptotic (annexin V+/PI-), and late apoptotic or necrotic (annexin V+/PI+) cells, and decreased active caspase-3 immunoreactivity. G-CSF could protect human cerebral neurons following in vitro ischemia.
MeSH terms
Blotting, WesternBrain Ischemia/pathology*Cell Death/drug effectsCell LineCell Survival/drug effectsFlow CytometryGranulocyte Colony-Stimulating Factor/pharmacology*HumansHybrid Cells/drug effectsL-Lactate Dehydrogenase/metabolismNecrosisNeurons/drug effects*Neuroprotective Agents*Tetrazolium SaltsThiazolesTranscription, Genetic/drug effects
DOI
10.1016/j.neulet.2005.10.040
PMID
16298488
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
AJOU Authors
김, 승업
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