Cited 0 times in Scipus Cited Count

OTUB1 knockdown promotes apoptosis in melanoma cells by upregulating TRAIL expression

DC Field Value Language
dc.contributor.authorLee, BS-
dc.contributor.authorKang, SU-
dc.contributor.authorHuang, M-
dc.contributor.authorKim, YS-
dc.contributor.authorLee, YS-
dc.contributor.authorPark, JY-
dc.contributor.authorKim, CH-
dc.date.accessioned2023-01-26T06:10:32Z-
dc.date.available2023-01-26T06:10:32Z-
dc.date.issued2021-
dc.identifier.issn1976-6696-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/24129-
dc.description.abstractMelanoma, the most serious type of skin cancer, exhibits a high risk of metastasis. Although chemotherapeutic treatmentfor metastatic melanoma improves disease outcome and patientsurvival, some patients exhibit resistance or toxicity to the drugtreatment regime. OTUB1 is a deubiquitinating enzyme overexpressedin several cancers. In this study, we investigated theeffects of inhibiting OTUB1 expression on melanoma-cell proliferationand viability and identified the underlying molecularmechanism of action of OTUB1. We did endogenous OTUB1knockdown in melanoma cells using short interfering RNA,and assessed the resulting phenotypes via MTT assays, Westernblotting, and cell-cycle analysis. We identified differentiallyexpressed genes between OTUB1-knockdown cells and controlcells using RNA sequencing and confirmed them via Westernblotting and reverse transcription polymerase chain reaction.Furthermore, we investigated the involvement of apoptotic andcell survival signaling pathways upon OTUB1 depletion. OTUB1depletion in melanoma cells decreased cell viability and causedsimultaneous accumulation of cells in the sub-G1 phase, indicatingan increase in the apoptotic-cell population. RNA sequencingof OTUB1-knockdown cells revealed an increase in thelevels of the apoptosis-inducing protein TRAIL. Additionally,OTUB1-knockdown cells exhibited increased sensitivity toPLX4032, a BRAF inhibitor, implying that OTUB1 and BRAFact collectively in regulating apoptosis. Taken together, ourfindings show that OTUB1 induces apoptosis of melanomacells in vitro, likely by upregulating TRAIL, and suggest thatapproaches targeting OTUB1 can be developed to provide noveltherapeutic strategies for treating melanoma.-
dc.language.isoen-
dc.subject.MESHApoptosis-
dc.subject.MESHApoptosis Regulatory Proteins-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHHumans-
dc.subject.MESHMelanoma-
dc.subject.MESHTNF-Related Apoptosis-Inducing Ligand-
dc.titleOTUB1 knockdown promotes apoptosis in melanoma cells by upregulating TRAIL expression-
dc.typeArticle-
dc.identifier.pmid34488924-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8728537/-
dc.subject.keywordCell death-
dc.subject.keywordMelanoma-
dc.subject.keywordOtub1-
dc.subject.keywordPlx4032-
dc.subject.keywordTrail-
dc.contributor.affiliatedAuthorKang, SU-
dc.contributor.affiliatedAuthorKim, CH-
dc.type.localJournal Papers-
dc.identifier.doi10.5483/BMBRep.2021.54.12.033-
dc.citation.titleBMB reports-
dc.citation.volume54-
dc.citation.number12-
dc.citation.date2021-
dc.citation.startPage608-
dc.citation.endPage613-
dc.identifier.bibliographicCitationBMB reports, 54(12). : 608-613, 2021-
dc.identifier.eissn1976-670X-
dc.relation.journalidJ019766696-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
Files in This Item:
34488924.pdfDownload

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse