33 365

Cited 75 times in

Brain transplantation of human neural stem cells transduced with tyrosine hydroxylase and GTP cyclohydrolase 1 provides functional improvement in animal models of Parkinson disease.

Authors
Kim, SU; Park, IH; Kim, TH; Kim, KS; Choi, HB; Hong, SH; Bang, JH; Lee, MA; Joo, IS; Lee, CS; Kim, YS
Citation
Neuropathology : official journal of the Japanese Society of Neuropathology, 26(2):129-140, 2006
Journal Title
Neuropathology : official journal of the Japanese Society of Neuropathology
ISSN
0919-65441440-1789
Abstract
Parkinson disease is a neurodegenerative disease characterized by loss of midbrain dopaminergic neurons resulting in movement disorder. Neural stem cells (NSC) of the CNS have recently aroused a great deal of interest, not only because of their importance in basic research of neural development, but also for their therapeutic potential in neurological disorders. We have recently generated an immortalized human NSC cell line, HB1.F3, via retrovirus-mediated v-myc transfer. This line is capable of self-renewal, is multipotent, and expresses cell specific markers for NSC, ATP-binding cassettes transporter (ABCG2) and nestin. Next, we co-transduced the F3 NSC line with genes encoding tyrosine hydroxylase (TH) and GTP cyclohydrolase 1 (GTPCH1) in order to generate dopamine-producing NSC. The F3.TH.GTPCH human NSC line expresses TH and GTPCH phenotypes as determined by RT-PCR, western blotting and immunocytochemistry, and shows a 800 to 2000-fold increase in production of L-dihydroxyphenyl alanine in HPLC analysis. A marked improvement in amphetamine-induced turning behavior was observed in parkinsonian rats implanted with F3.TH.GTPCH cells, but not in control rats receiving F3 NSC. In the animals showing functional improvement, a large number of TH-positive F3.TH.GTPCH NSC were found at injection sites. These results indicate that human NSC, genetically transduced with TH and GTPCH1 genes, have great potential in clinical utility for cell replacement therapy in patients suffering from Parkinson disease.
MeSH terms
AnimalsBlotting, WesternBrain Tissue Transplantation*Cell LineCell TransplantationChromatography, High Pressure LiquidDisease Models, AnimalDopamine/analysisDopamine/metabolismFemaleGTP Cyclohydrolase/geneticsGTP Cyclohydrolase/metabolismHumansImmunohistochemistryLevodopa/analysisLevodopa/metabolismMultipotent Stem Cells/transplantationNeurons/transplantation*Parkinson Disease/therapy*RatsRats, Sprague-DawleyReverse Transcriptase Polymerase Chain ReactionStem Cell Transplantation*Transduction, GeneticTyrosine 3-Monooxygenase/geneticsTyrosine 3-Monooxygenase/metabolism*
PMID
16708545
Appears in Collections:
Journal Papers > Research Organization > Brain Disease Research Center
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
Journal Papers > School of Medicine / Graduate School of Medicine > Brain Science
AJOU Authors
김승업박인호이명애주인수
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse