156 346

Cited 0 times in

Immunoglobulin can be functionally regulated by protein carboxylmethylation in Fc region.

Authors
Park, JS; Cho, JY; Kim, SS; Bae, HJ; Han, JW; Lee, HW; Hong, SY
Citation
Archives of pharmacal research, 29(5):384-393, 2006
Journal Title
Archives of pharmacal research
ISSN
0253-6269
Abstract
Protein carboxylmethylation methylates the free carboxyl groups in various substrate proteins by protein carboxyl O-methyltransferase (PCMT) and is one of the post-translational modifications. There have been many studies on protein carboxylmethylation. However, the precise functional role in mammalian systems is unclear. In this study, immunoglobulin, a specific form of gamma-globulin, which is a well-known substrate for PCMT, was chosen to investigate the regulatory roles of protein carboxylmethylation in the immune system. It was found that the anti-BSA antibody could be carboxylmethylated via spleen PCMT to a level similar to gamma-globulin. This carboxylmethylation increased the hydrophobicity of the anti-BSA antibody up to 11.4%, and enhanced the antigen-binding activity of this antibody up to 24.6%. In particular, the Fc region showed a higher methyl accepting capacity with 80% of the whole structure level. According to the amino acid sequence alignment, indeed, 7 aspartic acids and 5 glutamic acids, as potential carboxylmethylation sites, were found to be conserved in the Fc portion in the human, mouse and rabbit. The carboxylmethylation of the anti-BSA antibody was reversibly demethylated under a higher pH and long incubation time. Therefore, these results suggest that protein carboxylmethylation may reversibly regulate the antibody-mediated immunological events via the Fc region.
MeSH terms
Amino Acid SequenceAnimalsAntibodies/chemistryAntibodies/immunology*Antibodies/isolation & purificationBinding Sites, Antibody/immunologyImmunoglobulin Fab Fragments/chemistryImmunoglobulin Fab Fragments/immunologyImmunoglobulin Fc Fragments/chemistryImmunoglobulin Fc Fragments/immunology*Immunoglobulin G/chemistryImmunoglobulin G/immunology*MethylationMolecular Sequence DataPapainProtein O-Methyltransferase/chemistryProtein O-Methyltransferase/metabolism*RabbitsSequence AlignmentSerum Albumin, Bovine/immunology
PMID
16756083
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
AJOU Authors
김, 성수
Files in This Item:
Full-Text Not Available.txtDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse