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Loss of transgelin in repeated bouts of ulcerative colitis-induced colon carcinogenesis.

Authors
Yeo, M; Kim, DK; Park, HJ; Oh, TY; Kim, JH; Cho, SW; Paik, YK; Hahm, KB
Citation
Proteomics, 6(4):1158-1165, 2006
Journal Title
Proteomics
ISSN
1615-98531615-9861
Abstract
Though ulcerative colitis (UC)-associated colon cancer develops from dysplastic lesions caused by chronic inflammation, the specific mechanistic link between chronic inflammation and carcinogenesis in colon has not been integrated into molecular understanding. We therefore established an experimental animal model for colitic cancer, and used proteomic analysis, based on 2-DE and MALDI-TOF MS, to identify proteins involved in colitic cancer. In our model, 6-week-old C57BL/6J mice were exposed to 15 cycles of dextran sodium sulfate (DSS), with each cycle consisting of 0.7% DSS for 1 week followed by distilled water for 10 days. Colorectal tumors developed in 22 of 24 mice (91.6%), with a tumor multiplicity of 1.727 per tumor-bearing mouse. Comparative 2-DE analysis showed that 38 protein spots were differentially expressed in colon tumors and normal colon. We identified 27 of these proteins, including GRP94, HSC70, enolase, prohibitin, and transgelin. The reduction of transgelin expression in mouse colon tumors was confirmed by Western blotting and immunohistochemistry. We also found that transgelin expression was significantly reduced in human colon tumors compared with adjacent nontumorous tissues. In conclusion, these results suggest that loss of transgelin could be a candidate for biomarker of repeated colitis-associated colon cancer.
MeSH terms
AnimalsBlotting, WesternColitis, Ulcerative/chemically inducedColitis, Ulcerative/complicationsColitis, Ulcerative/metabolism*Colorectal Neoplasms/etiologyColorectal Neoplasms/metabolism*Dextran Sulfate/toxicityDown-RegulationFemaleMiceMice, Inbred C57BLMicrofilament Proteins/metabolism*Muscle Proteins/metabolism*ProteomicsSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
DOI
10.1002/pmic.200500390
PMID
16402363
Appears in Collections:
Journal Papers > Research Organization > Genomic Research Center for Gastroenterology
Journal Papers > School of Medicine / Graduate School of Medicine > Pathology
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
AJOU Authors
여, 말희김, 장희조, 성원함, 기백
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