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Detection of serum IgG autoantibodies to FcεRIα by ELISA in patients with chronic spontaneous urticaria

Jang, JH  | Moon, J | Yang, EM | Ryu, MS  | Lee, Y  | Ye, YM  | Park, HS
PloS one, 17(8). : e0273415-e0273415, 2022
Journal Title
PloS one
BACKGROUND: Mast cells are a key effector cell in the pathogenesis of chronic spontaneous urticaria (CSU) and activated by circulating FcepsilonRI-specific IgG as well as IgE. This study evaluated the prevalence of circulating autoantibodies to FcepsilonRIalpha in the sera of CSU patients. METHODS: Eighty-eight patients with CSU and 76 healthy controls (HCs) were enrolled. To detect circulating autoantibodies (IgG/IgA/IgM) to FcepsilonRIalpha, ELISA was done using YH35324 (as a solid phase antigen), and its binding specificity was confirmed by the ELISA inhibition test. The antibody levels were presented by the ratio of YH35324-preincubated to mock-preincubated absorbance values. Clinical and autoimmune parameters, including atopy, urticaria activity score (UAS), serum total/free IgE levels, serum antinuclear antibody (ANA) and autologous serum skin test (ASST) results, were assessed. The autoimmune group was defined if CSU patients had positive results to ASST and/or ANA. RESULTS: The ratio of serum IgG to FcepsilonRIalpha was significantly lower in CSU patients than in HCs (P<0.05), while no differences were noted in serum levels of IgG to recombinant FcepsilonRIalpha or IgA/IgM autoantibodies. The autoimmune CSU group had significantly lower ratios of IgG/IgA (not IgM) autoantibodies to FcepsilonRIalpha than the nonautoimmune CSU group (P<0.05 for each). No significant associations were found between sex, age, atopy, urticaria duration, UAS, or serum total/free IgE levels according to the presence of IgG/IgA/IgM antibodies. CONCLUSIONS: This study confirmed the presence of IgG to FcepsilonRIalpha in the sera of CSU patients, especially those with the autoimmune phenotype.

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Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Ajou Authors
박, 해심  |  예, 영민  |  유, 민숙  |  이, 영수  |  장, 재혁
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