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Claudin-1 acts through c-Abl-protein kinase Cdelta (PKCdelta) signaling and has a causal role in the acquisition of invasive capacity in human liver cells.

Authors
Yoon, CH; Kim, MJ; Park, MJ; Park, IC; Hwang, SG; An, S; Choi, YH; Yoon, G; Lee, SJ
Citation
The Journal of biological chemistry, 285(1):226-233, 2010
Journal Title
The Journal of biological chemistry
ISSN
0021-92581083-351X
Abstract
Claudins are identified as members of the tetraspanin family of proteins, which are integral to the structure and function of tight junction. Recent studies showed an increase in expression of claudins during tumorigenesis, which is associated with loss of cell-cell contact, dedifferentiation, and invasiveness. However, the molecular basis for the causal relationship between claudin expression and cancer progression is not fully understood yet. In this study, we show that claudin-1 plays a causal role in the acquisition of invasive capacity in human liver cells and that c-Abl-protein kinase Cdelta (PKCdelta) signaling is critical for the malignant progression induced by claudin-1. Overexpression of claudin-1 clearly induced expression of matrix metalloproteinase-2 (MMP-2) and cell invasion and migration in normal liver cells as well as in non-invasive human hepatocellular carcinoma (HCC) cells. Conversely, small interfering RNA targeting of claudin-1 in invasive HCC cells completely inhibited cell invasion. Both c-Abl and PKCdelta are found to be activated in normal liver cell line clones that stably overexpress claudin-1. Inhibition of either c-Abl or PKCdelta alone clearly attenuated MMP-2 activation and impeded cell invasion and migration in both human HCC and normal liver cells expressing claudin-1. These results indicate that claudin-1 is both necessary and sufficient to induce invasive behavior in human liver cells and that activation of c-Abl-PKCdelta signaling pathway is critically required for the claudin-1-induced acquisition of the malignant phenotype. The present observations raise the possibility of exploiting claudin-1 as a potential biomarker for the spread of liver cancer and might provide pivotal points for therapeutic intervention in HCC.
MeSH terms
Carcinoma, Hepatocellular/enzymologyCarcinoma, Hepatocellular/pathologyCell Line, TumorEnzyme ActivationHumansLiver/enzymology*Liver/pathology*Liver Neoplasms/enzymologyLiver Neoplasms/pathologyMembrane Proteins/metabolism*Neoplasm Invasiveness/pathology*Protein Kinase C-delta/metabolism*Proto-Oncogene Proteins c-abl/metabolism*Signal Transduction*
DOI
10.1074/jbc.M109.054189
PMID
19897486
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
AJOU Authors
윤, 계순
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