Cited 0 times in Scipus Cited Count

Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration

DC Field Value Language
dc.contributor.authorShin, MK-
dc.contributor.authorBang, JS-
dc.contributor.authorLee, JE-
dc.contributor.authorTran, HD-
dc.contributor.authorPark, G-
dc.contributor.authorLee, DR-
dc.contributor.authorJo, J-
dc.date.accessioned2023-02-21T04:33:50Z-
dc.date.available2023-02-21T04:33:50Z-
dc.date.issued2022-
dc.identifier.issn1661-6596-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/24726-
dc.description.abstractIn vitro organoids derived from human pluripotent stem cells (hPSCs) have been developed as essential tools to study the underlying mechanisms of human development and diseases owing to their structural and physiological similarity to corresponding organs. Despite recent advances, there are a few methodologies for three-dimensional (3D) skeletal muscle differentiation, which focus on the terminal differentiation into myofibers and investigate the potential of modeling neuromuscular disorders and muscular dystrophies. However, these methodologies cannot recapitulate the developmental processes and lack regenerative capacity. In this study, we developed a new method to differentiate hPSCs into a 3D human skeletal muscle organoid (hSkMO). This organoid model could recapitulate the myogenesis process and possesses regenerative capacities of sustainable satellite cells (SCs), which are adult muscle stem/progenitor cells capable of self-renewal and myogenic differentiation. Our 3D model demonstrated myogenesis through the sequential occurrence of multiple myogenic cell types from SCs to myocytes. Notably, we detected quiescent, non-dividing SCs throughout the hSkMO differentiation in long-term culture. They were activated and differentiated to reconstitute muscle tissue upon damage. Thus, hSkMOs can recapitulate human skeletal muscle development and regeneration and may provide a new model for studying human skeletal muscles and related diseases.-
dc.language.isoen-
dc.subject.MESHCell Differentiation-
dc.subject.MESHHumans-
dc.subject.MESHMuscle Development-
dc.subject.MESHMuscle, Skeletal-
dc.subject.MESHOrganoids-
dc.subject.MESHPluripotent Stem Cells-
dc.titleGeneration of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration-
dc.typeArticle-
dc.identifier.pmid35563499-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103168-
dc.subject.keywordhuman pluripotent stem cells-
dc.subject.keywordmyogene-sis-
dc.subject.keywordregeneration-
dc.subject.keywordsatellite cells-
dc.subject.keywordskeletal muscle organoids-
dc.contributor.affiliatedAuthorJo, J-
dc.type.localJournal Papers-
dc.identifier.doi10.3390/ijms23095108-
dc.citation.titleInternational journal of molecular sciences-
dc.citation.volume23-
dc.citation.number9-
dc.citation.date2022-
dc.citation.startPage5108-
dc.citation.endPage5108-
dc.identifier.bibliographicCitationInternational journal of molecular sciences, 23(9). : 5108-5108, 2022-
dc.identifier.eissn1422-0067-
dc.relation.journalidJ014220067-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
Files in This Item:
35563499.pdfDownload

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse