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Fibrillar superstructure formation of hemoglobin A and its conductive, photodynamic and photovoltaic effects.

Authors
Lee, JH; Kang, S; Lee, SG; Jin, JH; Park, JW; Park, SM; Jung, S; Paik, SR
Citation
Acta biomaterialia, 6(12):4689-4697, 2010
Journal Title
Acta biomaterialia
ISSN
1742-70611878-7568
Abstract
The fabrication of biomaterials which serve as functional scaffolds exhibiting diversified effects has been valued. We report here a unique strategy to fibrillate hemoglobin A (HbA), which exhibits multiple photoelectrochemical properties, and a subsequent specific defibrillation procedure. A subtle structural rearrangement of the α/β-subunits within the quaternary structure of HbA is responsible for the HbA fibril formation in the presence of 0.5% CHCl₃. The narrow pH dependence of the suprastructure formation around pH 7.4 illustrates the highly sensitive nature of the structural alteration. The CHCl₃-induced fibrils become disintegrated by ascorbic acid, indicating that the oxidation-reduction process of the iron within the heme moiety could be involved in stabilization of the fibrillar structures. The electron-transferring property of the iron allows the fibrils to exhibit not only their conductive behavior but also a photodynamic effect generating hydroxyl radicals in the presence of H(2)O(2) with light illumination. A photovoltaic effect is also demonstrated with the HbA fibrils, which generate an electric current on the fibril-coated microelectrode upon irradiation at 405nm. Taken together, the multiple effects of HbA fibrils and the selective fibrillation/defibrillation procedures could qualify the fibrils to be employed for various future applications in biotechnology, including bio-machine interfaces.
MeSH terms
Ascorbic Acid/pharmacologyChloroform/pharmacologyChromatography, High Pressure LiquidElectric Conductivity*ElectrodesElectrophoresis, Polyacrylamide GelHemoglobin A/chemistry*Hemoglobin A/ultrastructureHydrogen-Ion Concentration/drug effectsHydrogen-Ion Concentration/radiation effectsHydroxyl Radical/chemistryLight*Photochemical Processes/drug effectsPhotochemical Processes/radiation effects*Tin Compounds/chemistry
DOI
10.1016/j.actbio.2010.06.023
PMID
20601245
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
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