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The Nf-actin gene is an important factor for food-cup formation and cytotoxicity of pathogenic Naegleria fowleri.

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dc.contributor.authorSohn, HJ-
dc.contributor.authorKim, JH-
dc.contributor.authorShin, MH-
dc.contributor.authorSong, KJ-
dc.contributor.authorShin, HJ-
dc.date.accessioned2011-05-02T05:23:21Z-
dc.date.available2011-05-02T05:23:21Z-
dc.date.issued2010-
dc.identifier.issn0932-0113-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/2510-
dc.description.abstractNaegleria fowleri destroys target cells by trogocytosis, a phagocytosis mechanism, and a process of piecemeal ingestion of target cells by food-cups. Phagocytosis is an actin-dependent process that involves polymerization of monomeric G-actin into filamentous F-actin. However, despite the numerous studies concerning phagocytosis, its role in the N. fowleri food-cup formation related with trogocytosis has been poorly reported. In this study, we cloned and characterized an Nf-actin gene to elucidate the role of Nf-actin gene in N. fowleri pathogenesis. The Nf-actin gene is composed of 1,128-bp and produced a 54.1-kDa recombinant protein (Nf-actin). The sequence identity was 82% with nonpathogenic Naegleria gruberi but has no sequence identity with other mammals or human actin gene. Anti-Nf-actin polyclonal antibody was produced in BALB/c mice immunized with recombinant Nf-actin. The Nf-actin was localized on the cytoplasm, pseudopodia, and especially, food-cup structure (amoebastome) in N. fowleri trophozoites using immunofluorescence assay. When N. fowleri co-cultured with Chinese hamster ovary cells, Nf-actin was observed to localize around on phagocytic food-cups. We also observed that N. fowleri treated with cytochalasin D as actin polymerization inhibitor or transfected with antisense oligomer of Nf-actin gene had shown the reduced ability of food-cup formation and in vitro cytotoxicity. Finally, it suggests that Nf-actin plays an important role in phagocytic activity of pathogenic N. fowleri.-
dc.language.isoen-
dc.subject.MESHActins-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHAnimals-
dc.subject.MESHBase Sequence-
dc.subject.MESHCHO Cells-
dc.subject.MESHCloning, Molecular-
dc.subject.MESHCricetinae-
dc.subject.MESHCricetulus-
dc.subject.MESHCytoplasm-
dc.subject.MESHDNA, Protozoan-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred BALB C-
dc.subject.MESHMicroscopy, Fluorescence-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHNaegleria fowleri-
dc.subject.MESHPhagocytosis-
dc.subject.MESHProtozoan Proteins-
dc.subject.MESHPseudopodia-
dc.subject.MESHSequence Analysis, DNA-
dc.subject.MESHSequence Homology, Amino Acid-
dc.subject.MESHVirulence Factors-
dc.titleThe Nf-actin gene is an important factor for food-cup formation and cytotoxicity of pathogenic Naegleria fowleri.-
dc.typeArticle-
dc.identifier.pmid20143092-
dc.contributor.affiliatedAuthor김, 종현-
dc.contributor.affiliatedAuthor신, 호준-
dc.type.localJournal Papers-
dc.identifier.doi10.1007/s00436-010-1760-y-
dc.citation.titleParasitology research-
dc.citation.volume106-
dc.citation.number4-
dc.citation.date2010-
dc.citation.startPage917-
dc.citation.endPage924-
dc.identifier.bibliographicCitationParasitology research, 106(4). : 917-924, 2010-
dc.identifier.eissn1432-1955-
dc.relation.journalidJ009320113-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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