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An RNA-hydrolyzing recombinant antibody exhibits an antiviral activity against classical swine fever virus.

DC Field Value Language
dc.contributor.authorJun, HR-
dc.contributor.authorPham, CD-
dc.contributor.authorLim, SI-
dc.contributor.authorLee, SC-
dc.contributor.authorKim, YS-
dc.contributor.authorPark, S-
dc.contributor.authorKwon, MH-
dc.date.accessioned2011-05-02T05:33:35Z-
dc.date.available2011-05-02T05:33:35Z-
dc.date.issued2010-
dc.identifier.issn0006-291X-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/2515-
dc.description.abstractSome proteins with ribonuclease (RNase) activity have been shown to suppress viral replication. A well-characterized recombinant antibody, 3D8 single-chain variable fragment (3D8 scFv), has RNA-hydrolyzing and cell-penetrating activities. Here, we investigated antiviral activity of 3D8 scFv against classical swine fever virus (CSFV). In a cell line expressing 3D8 scFv (C26), intracellular RNA-hydrolysis activity was higher compared to control PK-15 cells and viral replication was strongly suppressed at the viral RNA level, with the evidence of independency of IFN-alpha/beta induction. Exogenous treatment of 3D8 scFv, prior to or post-CSFV infection, was also shown to suppress CSFV replication at the viral RNA level. These observations suggest that antiviral activity of 3D8 scFv may be due to the intrinsic RNase activity of 3D8 scFv, which is capable of targeting viral RNA genomes or transcripts.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHAntibodies, Antinuclear/metabolism-
dc.subject.MESHAntibodies, Antinuclear/pharmacology*-
dc.subject.MESHAntibodies, Monoclonal/metabolism-
dc.subject.MESHAntibodies, Monoclonal/pharmacology*-
dc.subject.MESHAntiviral Agents/metabolism-
dc.subject.MESHAntiviral Agents/pharmacology*-
dc.subject.MESHCell Line-
dc.subject.MESHClassical Swine Fever/immunology-
dc.subject.MESHClassical Swine Fever/virology*-
dc.subject.MESHClassical swine fever virus/drug effects*-
dc.subject.MESHDown-Regulation-
dc.subject.MESHHydrolysis-
dc.subject.MESHInterferon Type I/immunology-
dc.subject.MESHRNA, Viral/immunology-
dc.subject.MESHRNA, Viral/metabolism-
dc.subject.MESHRecombinant Proteins/metabolism-
dc.subject.MESHRecombinant Proteins/pharmacology*-
dc.subject.MESHRibonucleases/metabolism-
dc.subject.MESHRibonucleases/pharmacology-
dc.subject.MESHSingle-Chain Antibodies/immunology-
dc.subject.MESHSingle-Chain Antibodies/pharmacology*-
dc.subject.MESHSwine-
dc.subject.MESHVirus Replication/drug effects-
dc.titleAn RNA-hydrolyzing recombinant antibody exhibits an antiviral activity against classical swine fever virus.-
dc.typeArticle-
dc.identifier.pmid20382124-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0006-291X(10)00694-7-
dc.contributor.affiliatedAuthor박, 선-
dc.contributor.affiliatedAuthor권, 명희-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.bbrc.2010.04.032-
dc.citation.titleBiochemical and biophysical research communications-
dc.citation.volume395-
dc.citation.number4-
dc.citation.date2010-
dc.citation.startPage484-
dc.citation.endPage489-
dc.identifier.bibliographicCitationBiochemical and biophysical research communications, 395(4):484-489, 2010-
dc.identifier.eissn1090-2104-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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