Cited 0 times in Scipus Cited Count

Micrococcus luteus-derived extracellular vesicles attenuate neutrophilic asthma by regulating miRNAs in airway epithelial cells

DC Field Value Language
dc.contributor.authorSim, S-
dc.contributor.authorLee, DH-
dc.contributor.authorKim, KS-
dc.contributor.authorPark, HJ-
dc.contributor.authorKim, YK-
dc.contributor.authorChoi, Y-
dc.contributor.authorPark, HS-
dc.date.accessioned2023-05-04T06:41:43Z-
dc.date.available2023-05-04T06:41:43Z-
dc.date.issued2023-
dc.identifier.issn1226-3613-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/25307-
dc.description.abstractBacterial extracellular vesicles (EVs) have been shown to regulate various pulmonary diseases, but their functions in asthma remain uncertain. To demonstrate the clinical significance of Micrococcus luteus-derived EVs (MlEVs) in asthma, we enrolled 45 asthmatic patients (20 patients with neutrophilic asthma [NA], 25 patients with eosinophilic asthma [EA]) and 40 healthy controls (HCs). When the prevalence of IgG1 and IgG4 specific to MlEVs was evaluated in serum by ELISA, lower levels of MlEV-specific IgG4 (but not IgG1) were noted in asthmatic patients than in HCs. Among asthmatic patients, significantly lower levels of MIEV-specific IgG4 were noted in patients with NA than in those with EA. Moreover, there was a positive correlation between serum MlEV-specific IgG4 levels and FEV1 (%) values. In asthmatic C57BL/6 mice, MlEVs significantly attenuated neutrophilic airway inflammation by reducing the production of IL-1β and IL-17 in bronchoalveolar lavage fluid as well as the number of group 3 innate lymphoid cells (ILC3s) in lung tissues. To clarify the functional mechanism of MlEVs in NA, the effect of MlEVs on airway epithelial cells (AECs) and immune cells was investigated ex vivo. According to microarray analysis, MlEVs upregulated hsa-miR-4517 expression in AECs. Moreover, this miRNA could suppress IL-1β production by monocytes, resulting in the inhibition of ILC3 activation and neutrophil recruitment. These findings suggest that MlEVs could be a novel therapeutic agent for managing unresolved NA by regulating miRNA expression in AECs.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHAsthma-
dc.subject.MESHBronchoalveolar Lavage Fluid-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHEpithelial Cells-
dc.subject.MESHExtracellular Vesicles-
dc.subject.MESHImmunity, Innate-
dc.subject.MESHLymphocytes-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHMicrococcus luteus-
dc.subject.MESHMicroRNAs-
dc.titleMicrococcus luteus-derived extracellular vesicles attenuate neutrophilic asthma by regulating miRNAs in airway epithelial cells-
dc.typeArticle-
dc.identifier.pmid36639716-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898544-
dc.contributor.affiliatedAuthorChoi, Y-
dc.contributor.affiliatedAuthorPark, HS-
dc.type.localJournal Papers-
dc.identifier.doi10.1038/s12276-022-00910-0-
dc.citation.titleExperimental & molecular medicine-
dc.citation.volume55-
dc.citation.number1-
dc.citation.date2023-
dc.citation.startPage196-
dc.citation.endPage204-
dc.identifier.bibliographicCitationExperimental & molecular medicine, 55(1). : 196-204, 2023-
dc.identifier.eissn2092-6413-
dc.relation.journalidJ012263613-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Files in This Item:
36639716.pdfDownload

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse