Cited 0 times in Scipus Cited Count

Calcium ionophore-activated platelets induce eosinophil extracellular trap formation

DC Field Value Language
dc.contributor.authorSim, MS-
dc.contributor.authorKim, HJ-
dc.contributor.authorBae, I-
dc.contributor.authorKim, C-
dc.contributor.authorChang, HS-
dc.contributor.authorChoi, Y-
dc.contributor.authorLee, DH-
dc.contributor.authorPark, HS-
dc.contributor.authorChung, IY-
dc.date.accessioned2023-07-06T06:02:53Z-
dc.date.available2023-07-06T06:02:53Z-
dc.date.issued2023-
dc.identifier.issn1323-8930-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/26143-
dc.description.abstractBackground: Platelets play a modulatory role in inflammatory response by secreting a vast array of granules and disintegrating into membrane-bound microparticles upon activation. The interplay between eosinophils and platelets is postulated to be implicated in the pathology of allergic airway inflammation. In this study, we investigated whether activated platelets can induce eosinophil extracellular trap (EET) formation, a cellular process by which activated eosinophils release net-like DNA fibers. Methods: Platelets were stimulated with the calcium ionophore, A23187, and the platelet agonists, thrombin and adenosine diphosphate (ADP). Platelet cultures were fractionated into conditioned medium (CM) and pellet, which were then overlaid on eosinophils to examine EET formation. Results: The CM and pellet from A23187-activated platelets stimulated eosinophils to generate EET, whereas those from thrombin- or ADP-activated platelets failed to induce such generation. The EET-inducing activity of the A23187-activated platelet culture was linearly proportional to the number of activated platelets. Interestingly, while EET formation induced by the direct stimulation of eosinophils with A23187 was NADPH oxidase (NOX)-dependent, EET formation induced by A23187-activated platelets was NOX-independent and significantly inhibited by necroptosis pathway inhibitors. Conclusions: Activated platelets and their products may induce EET formation, thereby potentiating their role in eosinophilic airway inflammation.-
dc.language.isoen-
dc.subject.MESHAdenosine Diphosphate-
dc.subject.MESHBlood Platelets-
dc.subject.MESHCalcimycin-
dc.subject.MESHCalcium-
dc.subject.MESHCalcium Ionophores-
dc.subject.MESHExtracellular Traps-
dc.subject.MESHHumans-
dc.subject.MESHInflammation-
dc.subject.MESHThrombin-
dc.titleCalcium ionophore-activated platelets induce eosinophil extracellular trap formation-
dc.typeArticle-
dc.identifier.pmid36586745-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S1323893022001381?via%3Dihub-
dc.subject.keywordCalcium ionophore-
dc.subject.keywordEosinophil-
dc.subject.keywordEosinophil extracellular trap-
dc.subject.keywordPlatelet-
dc.subject.keywordThrombin-
dc.contributor.affiliatedAuthorChoi, Y-
dc.contributor.affiliatedAuthorPark, HS-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.alit.2022.12.002-
dc.citation.titleAllergology international-
dc.citation.volume72-
dc.citation.number3-
dc.citation.date2023-
dc.citation.startPage466-
dc.citation.endPage476-
dc.identifier.bibliographicCitationAllergology international, 72(3). : 466-476, 2023-
dc.identifier.eissn1440-1592-
dc.relation.journalidJ013238930-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Files in This Item:
36586745.pdfDownload

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse