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Eosinophil Activation in Severe Asthma
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 박, 해심 | - |
| dc.contributor.author | 이, 동현 | - |
| dc.date.accessioned | 2023-11-16T05:43:53Z | - |
| dc.date.available | 2023-11-16T05:43:53Z | - |
| dc.date.issued | 2023 | - |
| dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/26828 | - |
| dc.language.iso | en | - |
| dc.title | Eosinophil Activation in Severe Asthma | - |
| dc.title.alternative | 중증 천식에서 호산구 활성화 | - |
| dc.type | Thesis | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000032467 | - |
| dc.subject.keyword | Asthma | - |
| dc.subject.keyword | cytokeratin | - |
| dc.subject.keyword | eosinophil | - |
| dc.subject.keyword | epithelium | - |
| dc.subject.keyword | immunoglobulin G | - |
| dc.subject.keyword | extracellular traps | - |
| dc.subject.keyword | autoimmune response | - |
| dc.subject.keyword | interleukin-5 | - |
| dc.subject.keyword | antibody | - |
| dc.subject.keyword | 천식 | - |
| dc.subject.keyword | 사이토케라틴 | - |
| dc.subject.keyword | 호산구 | - |
| dc.subject.keyword | 상피 세포 | - |
| dc.subject.keyword | 면역글로불린 G | - |
| dc.subject.keyword | 세포외트랩 | - |
| dc.subject.keyword | 자가면역반응 | - |
| dc.subject.keyword | IL-5 | - |
| dc.subject.keyword | 수용체 항체 | - |
| dc.description.degree | Doctor | - |
| dc.contributor.department | 대학원 의생명과학과 | - |
| dc.contributor.affiliatedAuthor | 이, 동현 | - |
| dc.date.awarded | 2023 | - |
| dc.type.local | Theses | - |
| dc.citation.date | 2023 | - |
| dc.embargo.liftdate | 9999-12-31 | - |
| dc.embargo.terms | 9999-12-31 | - |
| dc.description.tableOfContents | CHAPTERⅠ 1
Ⅰ. INTRODUCTION 2 Ⅱ. MATERIALS AND METHODS 4 A. Study subjects and clinical parameters 4 B. Measurement of total IgG, CK18/CK19, and CK18/CK19-IgG Ab 4 C. Isolation of PBEs and EETs 5 D. Measurement of dsDNA and eosinophil granule protein 6 E. AEC culture and stimulation 6 F. In vivo experiments 6 G. Measurement of total IgG and CK18-IgG in mice 7 H. Western blotting analysis 8 I. Immunofluorescence analysis 8 J. Statistical analysis 9 Ⅲ. RESULTS 10 A. Clinical characteristics of the study subject 10 B. Levels of serum CK18 (but not CK19) were increased in patients with SA compared to those with NSA 12 C. Increased levels of serum CK18-specific IgG in patients with SA compared to those with NSA 13 D. Comparison of circulating CK18 levels according to asthma severity 14 E. Correlation between serum CK18- and CK19-specific IgG levels and lung function parameters 15 F. Degranulation of eosinophils and EETs increased the expression of epithelial autoantigen in AECs 16 G. CK18-specific IgG antibody induced eosinophil degranulation and EET formation 20 H. CK18/CK18-IgG production in the mouse model of SEA 22 I. Effect of EETs on CK18/CK18-IgG production in mice 25 Ⅳ. DISCUSSION 28 CHAPTER Ⅱ 32 Ⅰ. INTRODUCTION 33 Ⅱ. MATERIALS AND METHODS 35 A. Isolation of peripheral blood eosinophils (PBEs) and NK cells 35 B. Cell proliferation assays (CTG assays) 36 C. Ab-dependent cell–mediated cytotoxicity (ADCC) assays 36 D. Statistical analysis 37 Ⅲ. RESULTS 38 A. 5R65.7 significantly reduced eosinophil proliferation 38 B. 5R65.7 potently induced NK cell–mediated ADCC against eosinophils 40 Ⅳ. DISCUSSION 41 Ⅴ. CONCLUSIONS 43 REFERENCES 44 | - |
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