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Sirtuin 4 Isoform 1 Represses HBV Replication
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김, 경민 | - |
| dc.contributor.author | SAJJAD, MUHAMMAD AZHAR | - |
| dc.date.accessioned | 2023-11-16T05:43:56Z | - |
| dc.date.available | 2023-11-16T05:43:56Z | - |
| dc.date.issued | 2023 | - |
| dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/26844 | - |
| dc.language.iso | en | - |
| dc.title | Sirtuin 4 Isoform 1 Represses HBV Replication | - |
| dc.type | Thesis | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000033077 | - |
| dc.subject.keyword | Hepatitis B virus | - |
| dc.subject.keyword | HBV replication | - |
| dc.subject.keyword | Sirtuin 4 isoform 1 | - |
| dc.subject.keyword | SIRT4 catalytic inactive mutant | - |
| dc.description.degree | Master | - |
| dc.contributor.department | 대학원 의생명과학과 | - |
| dc.contributor.affiliatedAuthor | SAJJAD, MUHAMMAD AZHAR | - |
| dc.date.awarded | 2023 | - |
| dc.type.local | Theses | - |
| dc.citation.date | 2023 | - |
| dc.embargo.liftdate | 9999-12-31 | - |
| dc.embargo.terms | 9999-12-31 | - |
| dc.description.tableOfContents | I. INTRODUCTION 1
II. MATERIALS AND METHODS 4 1. Vector construction 4 2. Transfection and cell culture 6 3. Core particle and immunoblotting 7 4. Northern and southern blottings 7 5. SDS-PAGE and Western blotting 8 6. Co-immunoprecipitation 9 7. Statistical analysis 9 III. RESULTS 10 1. SIRT4 expression in HBV-associated hepatocellular carcinoma (HCC) tumor and adjacent non-tumor tissues 10 2. Isoforms of SIRT4 12 3. HBV replication upregulates the endogenous SIRT4 protein 14 4. Overexpression of SIRT4.1 downregulates HBc protein level, core particle formation, and HBV RI DNA synthesis 16 5. Overexpression of SIRT4.1 downregulates HBV mRNA synthesis 18 6. Overexpression effect of catalytically inactive SIRT4.1 (H161Y) mutant on HBc protein level, core particle formation, and HBV RI DNA synthesis 20 7. Effect of overexpression of catalytically inactive SIRT4.1 (H161Y) mutant on HBV mRNA synthesis 22 8. SIRT4.1 acts as upregulator of AMPK 25 9. SIRT4.1 binds to AMPK 27 IV. DISCUSSION 30 V. REFERENCES 33 | - |
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