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Structurally constrained peptides prevent SARS-CoV-2 spike – hACE2 interaction and neutralize pseudovirus in vitro.
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 우, 현구 | - |
| dc.contributor.author | TRINH, THANH THAO | - |
| dc.date.accessioned | 2023-11-16T05:43:56Z | - |
| dc.date.available | 2023-11-16T05:43:56Z | - |
| dc.date.issued | 2023 | - |
| dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/26846 | - |
| dc.language.iso | en | - |
| dc.title | Structurally constrained peptides prevent SARS-CoV-2 spike – hACE2 interaction and neutralize pseudovirus in vitro. | - |
| dc.type | Thesis | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000033107 | - |
| dc.subject.keyword | Neutralizing peptides | - |
| dc.subject.keyword | SARS-CoV-2 | - |
| dc.subject.keyword | variants of concern | - |
| dc.subject.keyword | infection | - |
| dc.subject.keyword | human ACE2 | - |
| dc.description.degree | Master | - |
| dc.contributor.department | 대학원 의생명과학과 | - |
| dc.contributor.affiliatedAuthor | TRINH, THANH THAO | - |
| dc.date.awarded | 2023 | - |
| dc.type.local | Theses | - |
| dc.citation.date | 2023 | - |
| dc.embargo.liftdate | 9999-12-31 | - |
| dc.embargo.terms | 9999-12-31 | - |
| dc.description.tableOfContents | BACKGROUND 1
1. The pandemic 1 2. SARS-CoV-2 1 a. Biology of SARS-CoV-2 1 b. Cell entry of SARS-CoV-2 2 c. Evolution of SARS-CoV-2 and Spike mutation 4 3. SARS-CoV-2 spike neutralization. 5 MATERIALS AND METHODS 7 Neutralizing peptides designing process and synthesis. 7 Surface plasmon resonance (SPR) analysis 7 Cell culturing 7 Transient transfection 8 Pseudovirus particles production 8 Fluorescence microscope 9 qPCR 9 Pseudovirus-based neutralizing assay (Luciferase assay) 10 Cell viability assay 10 Proteinase K assay 10 RESULTS 12 1. Candidates for neutralizing peptides 12 2. The neutralizing peptides are not toxic to the cells. 15 3. Human ACE2 expressing cell line construction. 16 4. Neutralize effects of peptides on different variants 18 5. The peptides work in dose-dependent manners. 20 6. Stability of neutralizing peptides 22 DISCUSSION 23 CONCLUSION 25 REFERENCES 26 | - |
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